AdvanceBio Amide HILIC & Glycan Mapping Columns from Agilent
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AdvanceBio Glycan Mapping HPLC Columns from Agilent
Agilent AdvanceBio Glycan Mapping Columns are designed and manufactured to deliver fast, high resolution, reproducible glycan identification using HILIC chromatography. AdvanceBio Glycan Mapping columns leverage technology that optimizes results for MS and fluorescence detection.
Choose from two UHPLC configurations: 2.7 µm superficially porous, for high resolution and lower backpressure, or 1.8 µm for highest resolution.
Order AdvanceBio Glycan Mapping columns with a selection of standards for performance testing and retention mapping of labeled and unlabeled glycans.
Our AdvanceBio N-glycan sample preparation kits provide all the components needed for the sample prep workflow with a comprehensive procedure to simplify the process.
Agilent's AdvanceBio Amide HILIC and Glycan Mapping columns are designed to provide fast, high-resolution, and reproducible glycan identification using hydrophilic interaction liquid chromatography (HILIC). These columns are optimised for both MS and fluorescence detection, delivering glycan profiles in under 10 minutes - 40% faster than competing products. They are also available with optional standards for performance testing and retention mapping of both labeled and unlabeled glycans.
The latest offering, Agilent’s AdvanceBio Amide HILIC columns, deliver maximum separation performance for glycan analysis, featuring enhanced peak capacity and improved temperature stability compared to the legacy Glycan Mapping columns. These Amide HILIC columns come with 1.8 µm particles and are available in 100 mm and 150 mm lengths.
The legacy Glycan Mapping columns are offered in two configurations: 2.7 µm superficially porous particles for high resolution with lower backpressure, and 1.8 µm particles for the highest resolution.
Features
- Achieve glycan profiles in under 10 minutes, delivering results 40% faster than competitors
- Accurately characterise both labelled and unlabelled glycans, and detect glycosylation changes caused by process variations
- Quality assured through testing with 2-AB- or InstantPC-labeled N-linked glycan samples, ensuring consistent performance
- AdvanceBio Amide HILIC columns offer exceptional long-term stability, even at high temperatures up to 80°C
- N-Glycan sample preparation kits provide all reagents needed to prepare samples before chromatographic analysis
Specification
| AdvanceBio Amide HILIC | AdvanceBio Glycan Mapping | ||
| Particle Size | 1.8 µm | 1.8 µm | 2.7 µm |
| Particle Type | Fully porous | Fully porous | Superficially porous |
| Bonded Phase | Amide HILIC | Amide HILIC | Amide HILIC |
| Pore Size | 300 Å | 300 Å | 120 Å |
| Temperature Limit | 80°C | 40°C | 40°C |
| pH Range | 2 - 7 | 2 - 7 | 2 - 7 |
| Pressure Limit | 1200 bar | 1200 bar | 600 bar |
| Hardware | Stainless Steel | Stainless Steel | Stainless Steel |
