Agilent Bio IEX Columns
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Agilent Bio IEX HPLC Columns
Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX) and weak anion-exchange (WAX) phases. All Bio Ion-Exchange phases are available in 1.7, 3, 5 and 10 µm non-porous particles sizes.
Agilent Bio IEX columns are packed with polymeric, nonporous, ion-exchange particles and are designed for high resolution, high recovery, and highly efficient separations of peptides, oligonucleotides, and proteins. The Bio IEX family includes strong cation-exchange (SCX), weak cation-exchange (WCX), strong anion-exchange (SAX), and weak anion-exchange (WAX) phases. All phases are available in 1.7, 3, 5, and 10 μm nonporous particles. PEEK-lined hardware is available for the 2.1 mm and 4.6 mm ID columns with 5 µm and 10 µm particle sizes for compounds that are sensitive to metal.
Features
- Highly crosslinked and rigid nonporous poly(styrene divinylbenzene) (PS/DVB) particles are grafted with a hydrophilic, polymeric layer, eliminating nonspecific binding
- Uniform, densely packed ion-exchange functional groups are chemically bonded to the hydrophilic layer (multiple ion-exchange groups per anchoring) to increase column capacity
- Particles, coating and bonding are resistant to high pressures, promoting higher resolution and faster separations
- Multiple ion-exchange groups are captured on one anchoring to increase capacity
Specification
| Agilent Bio IEX | |
| Phases | SCX (Strong cation-exchange) – SO3H WCX (Weak cation-exchange) – COOH SAX (Strong anion-exchange) – N(CH3)3 WAX (Weak anion-exchange) – N(C2H5)2 |
| Particle Type | Non-porous |
| Particle Size | 1.7, 3, 5 and 10 µm |
| pH Range | 2 - 12 |
| Maximum Temperature | 80°C |
| Hardware | Stainless Steel (1.7 µm, 3 µm, 5 µm, 10 µm) PEEK (5 µm, 10 µm) |
