Prep and Zorbax PrepHT Columns from Agilent
All pictures shown are for illustration purpose only. Actual product may vary.
Agilent Prep LC Columns are designed for high loadability to purify milligram to gram quantities of products. Column dimensions are available for most preparative sample types ranging from high throughput samples generated in drug discovery to lab scale prep and up to some pilot scale sample preparation. Preparative sized columns are available in 10–50 mm internal diameters with lengths ranging from 50–250 mm. Columns are available in 5, 7 and 10 µm particle sizes with very high efficiency in every dimension. Analytical size 4.6 mm id Scalar columns are available for method development and optimization prior to scaling up to larger columns. Agilent Prep columns are available in a C18 bonded phase suitable for purification of a wide variety of non-polar and polar compounds. Unbonded silica columns are also available.
Zorbax optimized phases are now available in preparative dimensions as PrepHT columns. These columns are 21.2 mm in diameter with 5 µm and 7 µm particles to provide high resolving power. Zorbax PrepHT columns have a broad range of chemistries, allowing you to maintain selectivity of the analytical phase in your prep separations. Zorbax PrepHT are available in a variety of bonded phases - Eclipse XDB, StableBond, Bonus-RP and Extend-C18 - for optimized resolution and loadability under any conditions.
Features
Prep Columns and Cartridges:
- Agilent prep columns and cartridges are available in C18 and Silica phases
- Cartridges are available with 21.1mm id, and columns have id options between 10 and 50mm
- Scalar columns are also available, giving easy scalability from 4.6 mm id to larger ids for rapid method development
- Exceptional column stability up to pH 10 (C18 pH 2 - 10, Silica pH 1 - 8)
- High loadability for maximum sample purification
- Maximum operating pressure is 340 bar for columns and cartridges, and 400 bar for Scalar columns
- 5 and 10 µm particles for high efficiency and high yield
- Pore sizes are 100 Å
- Lengths are available between 50 and 250mm
Zorbax PrepHT Cartridges:
- Widce range of phases available including: C18, C8, C3, Silica, Bonus RP, Cyano, Amino, Phenyl and Aq
- Variety of bonded phases - Eclipse XDB, StableBond, Bonus-RP and Extend-C18 - for optimized resolution and loadability under any conditions.
- Wide pore 300 Å Stable Bond phases also available.
- Easy to install finger-tight connections seal up to 5000 psi/350 bar
- Fast preparative separations, up to 2000 mg
- 5 and 7 µm particles for high efficiency and high yield
- Lengths are available between 50 and 250mm
- Cartridges are available with 21.1mm id
Specifications
Prep Columns and Cartridges:
|
|
Pore Size |
Surface Area |
Temperature Limits |
pH Range |
Endcapped |
Carbon Load |
|
C18 |
100 Å |
400 m2/g |
60 °C 1 |
2 - 10 |
Yes |
24% |
|
Silica |
100 Å |
400 m2/g |
2 |
1 - 8 |
No |
- |
1 Temperature limits are 60 °C for pH 2 - 8, 40 °C for pH 8 - 10
2 Temperature limits for bare silica are determined by the pH of the mobile phase
Zorbax Prep HT Cartridges:
|
|
Pore Size |
Surface Area |
Temperature Limits |
pH Range |
Endcapped |
Carbon Load |
|
ODS |
70 Å |
|
60 °C |
2 - 8 |
Yes |
20% |
|
Rx-C18 |
80 Å |
180 m2/g |
60 °C 4 |
2 - 8 |
No |
12% |
|
XDB-C18 |
80 Å |
180 m2/g |
60 °C 2 |
2 - 9 |
Double |
10% |
|
Extend C18 |
80 Å |
180 m2/g |
60 °C 3 |
2 - 11.5 |
Double |
12.5% |
|
C8 |
70 Å |
180 m2/g |
60 °C |
2 - 8 |
Yes |
12% |
|
XDB-C8 |
80 Å |
|
60 °C 2 |
2 - 9 |
Double |
7.6% |
|
Bonus-RP |
80 Å |
180 m2/g |
60 °C 1 |
2 - 9 |
Triple |
9.5% |
|
Rx-Silica |
80 Å |
180 m2/g |
5 |
0.8 - 8 |
No |
- |
|
Silica |
70 Å |
300 m2/g |
5 |
0.8 - 8 |
No |
- |
|
CN |
70 Å |
300 m2/g |
60 °C |
2 - 7 |
Yes |
7% |
|
NH2 |
70 Å |
300 m2/g |
60 °C |
2 - 7 |
Yes |
4% |
|
SB-C18 |
80 Å |
180 m2/g |
90 °C 6 |
0.8 - 8 |
No |
10% |
|
SB-C8 |
80 Å |
180 m2/g |
80 °C 7 |
1 - 8 |
No |
5.5% |
|
SB-Phenyl |
80 Å |
180 m2/g |
80 °C 7 |
1 - 8 |
No |
5.5% |
|
SB-CN |
80 Å |
180 m2/g |
80 °C 7 |
1 - 8 |
No |
4% |
|
SB-Aq |
80 Å |
180 m2/g |
80 °C 7 |
1 - 8 |
No |
proprietary |
|
300SB-C18 |
300 Å |
|
90 °C |
1 - 8 |
|
|
|
300SB-C8 |
300 Å |
|
80 °C |
1 - 8 |
|
|
|
300SB-C3 |
300 Å |
|
80 °C |
1 - 8 |
|
|
|
300SB-CN |
300 Å |
|
80 °C |
1 - 8 |
|
|
1 Temperature limits are 40 °C for pH 8 - 9, 60 °C for pH 2 - 8
2 Temperature limits are 40 °C for pH 6 - 9, 60 °C for pH 2 - 6
3 Temperature limits are 40 °C for pH 8 - 11.5, 60 °C for pH 2 - 8
4 Temperature limits are 40 °C for pH 6 - 8, 60 °C for pH 2 - 6
5 Temperature limits for bare silica are determined by the pH of the mobile phase
6 Temperature limits are 40 °C for pH 6 - 8, 90 °C for pH 1 - 6
7 Temperature limits are 40 °C for pH 6 - 8, 80 °C for pH 1 - 6
When using silica-based columns at pH > 6, best column lifetime is obtained at lower temperatures (40 °C max) using low buffer concentrations in the range of 0.01 to 0.02 M. Operating at extreme ends of pH and temperature ranges will have a significant impact on column lifetime.
Literature
Overview of Agilent's range of prep columns for small molecules and biomolecules