Hi-Plex HPLC Columns from Agilent

Agilent's Hi-Plex HPLC column provides reliable carbohydrate analysis with simple instrument requirements. Unlike Ion Chromatography columns, Hi-Plex columns use pure water or dilute acid on a standard HPLC, and do not require ion regeneration or suppressors. The media consists of robust a sulfonated polystyrene gel impregnated with various metal cations with different selectivities, as described in USP L17, L19, L34, and L58.

Part Number Product Description Price Qty
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Features

  • Agilent's recommended column for accurate analysis of carbohydrates
  • Operates on a standard HPLC and does not require specialized equipment
  • Long column life and excellent batch-to-batch reproducibility
  • Wide range of counterions and column configurations to optimize resolution
  • Simple operating conditions: water or dilute acid, isocratic conditions, up to 30 % ACN as a modifier
  • Available in 8 µm and 10 µm particle sizes
  • Complies with USP L17, USP L19, USP L34, and USP L58

Specifications

Bonded Phase Temperature Range Flow Rate Eluent USP Designation
Hi-Plex Ca 80-90°C 0.6 mL/min Water L19
Hi-Plex Ca USP L19 80-90°C 0.3 mL/min Water L19
Hi-Plex Pb 70-90°C 0.6 mL/min Water L34
Hi-Plex H for carbohydrates 60-70°C 0.6 mL/min Water L17
Hi-Plex H for organic acids 40-60°C 0.6 mL/min Dilute Acid L17
Hi-Plex Ca (Duo) 80-90°C 0.6 mL/min Water L19
Hi-Plex K 80-90°C 0.6 mL/min Water -
Hi-Plex Na (Octo) 80-90°C 0.6 mL/min Water, Sodium Hydroxide L58
Hi-Plex Na 80-90°C 0.3 mL/min Water L58

Literature

Hi-Plex Brochure (486.38 kB)
Confident, lower-pressure analysis of carbohydrates, alcohols, and organic acids
Analysis of carbohydrates, alcohols, and organic acids