Agilent Zorbax Ion Exchange Columns

ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides.

ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability. Therefore, they can be used at high operating flow rates and pressures. The columns are compatible with rapid changes in ionic strength, thus short gradient times and rapid re-equilibration are possible.

ZORBAX SAX packing has a permanently bonded quaternary amine. A trifunctional organo-silane reagent is used in producing this packing to maximize its stability with aqueous mobile phases. This column is ideal for separation of water-soluble compounds such as aromatic and aliphatic carboxylic acids and sulfonic acids.

ZORBAX SCX packing has 300 Å pore size silica particles chemically bonded to an aromatic sulfonic acid group. This column is used for separations of basic, water-soluble compounds and bio-molecules.

Agilent ZORBAX Ion Exchange columns are available as both strong anion exchange (SAX) and strong cation exchange (300SCX) columns. Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides, and polynucleotides.

ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability and so they can be used at high operating flow rates and pressures. The columns are compatible with rapid changes in ionic strength, thus short gradient times and rapid re-equilibration are possible.

ZORBAX SAX packing has a permanently bonded quaternary amine with a 70 Å pore size. It is best suited for small molecule separations of water-soluble compounds such as aromatic and aliphatic carboxylic acids and sulfonic acids.

ZORBAX SCX packing has 300 Å pore size silica particles chemically bonded to an aromatic sulfonic acid group. This column is used for separations of basic, water-soluble compounds and biomolecules.

Part Number Product Description Price Qty
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Features

  • ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica
  • Stable from pH 2–7
  • Provide high efficiency, rapid separations
  • Compatible with organic mobile phase modifiers

Specifications

  ZORBAX SAX ZORBAX 300SCX
Functionality Quaternary amine Sulfonic acid
Particle Type Fully Porous Fully Porous
Particle Size 5 µm 5 µm
Pore Size 70 Å 300 Å
Surface Area 300 m2/g 50 m2/g
pH Range 2 - 7 2 - 7
Maximum Temperature 80°C 80°C
Maximum Pressure 350 bar 350 bar
Hardware Stainless Steel Stainless Steel
USP Designation L14 L9

Literature

Your essential resource for biomolecule analysis