YMC BioPro IEX Columns
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BioPro IEX Biochromatography Columns from YMC
Ion exchange chromatography (IEX) is widely used for analysis and purification of biomolecules. BioPro IEX columns are specifically designed for separation of proteins, peptides and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They also show higher binding capacity and higher recovery of biomolecules compared to conventional IEX columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes!
Available phases are:
BioPro IEX QA - porous polymer beads with -CH2N+(CH3)3 charged group
BioPro IEX SP - porous polymer beads with -(CH2)3SO3- charged group
BioPro IEX QF - non-porous polymer beads with -CH2N+(CH3)3 charged group
BioPro IEX SF - non-porous polymer beads with -(CH2)3SO3- charged group
YMC BioPro IEX columns are widely used for the analysis and purification of biomolecules and are specifically designed for the separation of proteins, peptides, and nucleic acids.
BioPro IEX columns are based on porous and non-porous hydrophilic polymer beads with low nonspecific adsorption. They show higher binding capacity and higher recovery of biomolecules compared to conventional ion exchange columns. The completely spherical, monodisperse beads, together with optimal packing technology, provide high theoretical plate numbers and symmetrical peak shapes.
Available phases are:
BioPro IEX QA - porous strong anion exchanger
BioPro IEX SP - porous strong cation exchanger
BioPro IEX QF - non-porous strong anion exchanger
BioPro IEX SF - non-porous strong cation exchanger
Features
- Non-porous or porous particles
- Very high resolution
- Long term stability
- High loadability and recovery
- High dynamic binding capacity
- Excellent lot-to-lot reproducibility
Specifications
| BioPro IEX QA | BioPro IEX SP | BioPro IEX QF | BioPro IEX SF | |
| Matrix | Hydrophilic polymer (polymethacrylate) | Hydrophilic polymer (polymethacrylate) | Hydrophilic polymer (polymethacrylate) | Hydrophilic polymer (polymethacrylate) |
| Particle Size | 5 µm | 5 µm | 3, 5 µm | 3, 5 µm |
| Pore Size | 100 nm / 1000 Å | 100 nm / 1000 Å | Non-porous | Non-porous |
| Charged Group | -CH2N+(CH3)3 (strong anion exchanger) |
-(CH2)3SO3- (strong cation exchanger) |
-CH2N+(CH3)3 (strong anion exchanger) |
-(CH2)3SO3- (strong cation exchanger) |
| Counter Ion | Cl- | Na+ | Cl- | Na+ |
| Ion-exchange Capacity | 0.075 - 0.100 meq/mL-resin | 0.070 - 0.095 meq/mL-resin | 0.075 - 0.110 meq/mL-resin | 0.230 - 0.290 meq/mL-resin |
| Dynamic Binding Capacity | 110 - 150 mg BSA/mL-resin | 70 - 100 mg human IgG/mL-resin | 12 - 20 mg BSA/mL-resin | 10 - 18 mg human IgG/mL-resin |
| pH Range | 2 - 12 | 2 - 12 | 2 - 12 | 2 - 12 |
| Temperature Range | 4 - 60°C | 4 - 60°C | 4 - 60°C | 4 - 60°C |
| Column Hardware | PEEK | PEEK | PEEK | PEEK |
