Agilent ZORBAX 300Extend-C18 Columns
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Zorbax 300Extend-C18 HPLC Columns from Agilent
Zorbax 300Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5. Extend-C18 and 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH. At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge.
Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18. In addition, LC-MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase. These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18. The Zorbax 300Extend-C18 is a rugged column and is ideal for LC-MS with ammonium hydroxide mobile phase.
Agilent ZORBAX 300Extend-C18 is a wide-pore HPLC column for high-efficiency separations of peptides from pH 2 - 11.5. The unique, bidentate bonded phase provides excellent lifetime and reproducibility at high and low pH. At high pH, retention and selectivity of peptides and polypeptides can change dramatically because of changes in charge on molecules. Excellent recoveries of hydrophobic polypeptides have been achieved at room temperature and high pH. LC/MS sensitivity of peptides and polypeptides can also be improved at high pH using a simple ammonium-hydroxide containing mobile phase.
Features
- Rugged, high and low pH separations of peptides, polypeptides, and small proteins from pH 2 - 11.5
- Different selectivity possible at high and low pH
- High efficiency and good recovery of hydrophobic peptides at high pH
- Ideal for LC-MS with ammonium-hydroxide-modified mobile phase
Specifications
| ZORBAX 300Extend-C18 | |
| Bonded Phase | C18 |
| Particle Type | Fully Porous |
| Endcapped | Yes |
| Carbon Load | 4% |
| Particle Size | 3.5 µm, 5 µm |
| Pore Size | 300 Å |
| Temperature Limit | 60 °C up to pH 8, 40 °C from pH 8 - 11.5 |
| pH Range | 2 - 11.5 |
| USP Designation | L1 |
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