HALO® BioClass Diphenyl Columns from Advanced Materials Technology

HALO® Bioclass Diphenyl columns from Advanced Materials Technology come in two pore sizes of 400 Å and 1000 Å and are a valuable addition to the protein chemist’s separation toolbox for mAb realease assays and characterization methods. The HALO® Diphenyl offers a unique selectivity compared to C4 and C18 and demonstrates excellent stability with good sample recovery.

The 400 Å pore size column has a 3.4 µm superficially porous particle, whereas the 1000 Å pore size column has a 2.7 µm superficially porous particle. The columns provide narrow peak shapes and better sample recoveries for large biomolecules that range from 2 kDa and higher when compared to smaller pore sizes and fully porous particles.

Part Number Product Description Price Qty
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Features

  • HALO® 400 Å Diphenyl is ideal for large protein analysis and well suited for high speed protein separations
  • HALO® 1000 Å Diphenyl is suitable for mAb analysis and offers best in class resolution for full characterization
  • Alternate selectivity to HALO® C4 and HALO® ES-C18 columns
  • USP designation of L11
  • Available particle sizes of 2.7 µm for 1000 Å pore size and 3.4 µm for 400 Å pore size

Specifications

  HALO® 400 Å Diphenyl HALO® 1000 Å Diphenyl
Phase diphenylmethyl diphenylmethyl
Particle Type Fused-Core®  Fused-Core® 
Particle Size 3.4 µm 2.7 µm
Pore Size 400 Å 1000 Å
Endcapped Yes Yes
Low pH/Temperature Limit 2/90°C 2/90°C
High pH/Temperature Limit 9/40°C 9/40°C
Hardware Stainless Steel Stainless Steel
USP Designation L11 L11

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Literature

A better path to separations with Fused-Core®
HALO® columns for protein, peptide and glycan applications