AdvanceBio SEC Columns from Agilent
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AdvanceBio SEC HPLC Columns from Agilent
AdvanceBio SEC columns deliver accurate, precise quantitation for mAb aggregation and protein analysis. These innovative size exclusion chromatography columns were designed and engineered by Agilent to improve lab productivity by providing robust, reliable methods that eliminate sample reanalysis. Consistent results are achieved from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.
The AdvanceBio SEC 1.9 µm column is designed to improve resolution and provided faster separation of mAb aggregates and fragments by using both HPLC and UHPLC. The optimized sub-two-micron particles are monodispersed and modified by unique hydrophilic bonding chemistry providing column reproducibility and stability, as well as preventing secondary interactions and protein adsorption. The AdvanceBio SEC 1.9 µm column is intended to meet the need for qualitative and quantitative critical quality attributes (CQA) analysis and method transferability across departments.
AdvanceBio SEC columns from Agilent provide precise and accurate quantitation for analysing aggregates and fragments in peptides, monoclonal antibodies, and larger biotherapeutics. These size exclusion chromatography columns enhance laboratory efficiency by offering robust, reliable methods that prevent the need for retesting samples. They deliver consistent results across different columns, batches, and laboratories, facilitating method transfer across various departments and sites, and in doing so reducing uncertainty.
The AdvanceBio SEC 2.7 µm columns are designed for routine SEC applications and feature a unique hydrophilic coating that reduces secondary interactions with the sample and the stationary phase. This makes them particularly effective for challenging samples like antibody-drug conjugates (ADCs), providing reliable and precise results. Originally available in 300 Å and 130 Å pore sizes, these columns are optimal for sensitive and reproducible analysis of protein and peptide aggregates. The latest AdvanceBio SEC 2.7 µm columns now come in larger pore sizes of 500 Å and 1000 Å, tailored for high-resolution aggregate analysis of adeno-associated viruses (AAVs) and virus-like particles (VLPs).
AdvanceBio SEC 1.9 µm columns elevate SEC separations further. Their smaller particle size and hydrophilic properties allow for high-efficiency, high-resolution separations. Capable of withstanding pressures up to 620 bar, these columns support faster flow rates, meeting high-throughput demands. The 200 Å pore size is specially optimised for the separation of dimers, monomers, and fragments, ensuring comprehensive monoclonal antibody (mAb) characterisation.
Features
- High resolution, high-efficiency separation, and accurate quantitation for aggregates and fragments analysis
- Prevented secondary interactions with mAb, ADC, and other proteins
- Assured consistent column performance to eliminate rework
- Enhanced column lifetime and productivity to meet robust CQA analysis
- Specially designed standards for AdvanceBio SEC columns, providing optimal calibration and performance verification
- AdvanceBio SEC 300 Å 2.7 µm is intended for separation of proteins ranging from 5,000 to 1.25 million Da including mAb fragments, mAb monomer, dimer, and high-level aggregates
- AdvanceBio SEC 130 Å 2.7 µm is designed for small therapeutics with molecular weight ranging from 100 to 120,000 Da
- AdvanceBio SEC 500 Å is recommended for aggregate analysis of large biomolecules such as AAVs and VLPs in the range 5000 Da - 5 MDa
- AdvanceBio SEC 1000 Å is designed for analysis of large biological molecules such as VLPs and large oligonucleotides with molecular weight range of 15,000 Da to 10 MDa
- AdvanceBio SEC 200 Å 1.9 µm is suited for analysing protein ranging from 2,000 to 700,000 Da including mAb monomer, dimmer, and LMW protein fragments
- AdvanceBio SEC 120 Å 1.9 µm is best suited for analysis of peptides and small therapeutic proteins in the range 100 to 80,000 Da
Specifications
| AdvanceBio SEC | ||||||
| Pore Size | 120 Å | 130 Å | 200 Å | 300 Å | 500 Å | 1000 Å |
| Particle size | 1.9 µm | 2.7 µm | 1.9 µm | 2.7 µm | 2.7 µm | 2.7 µm |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 1000 Da | 100 Da | 2000 Da | 5000 Da | 5000 Da | 15,000 Da |
| Molecular Weight Upper Limit | 80,000 Da | 120,000 Da | 700,000 Da | 1,250,000 Da | 5,000,000 Da | 10,000,000 Da |
| Temperature Limit | 80°C | 80°C | 80°C | 80°C | 80°C | 80°C |
| pH range | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 | 2 - 8.5 |
