SiELC BIST A HPLC Columns
All pictures shown are for illustration purpose only. Actual product may vary.
SiELC BIST A is a negatively charged silica-based cation-exchange HPLC column with hydrophilic properties. Retention is achieved by using a multi-charged positive buffer, which acts as a bridge, linking negatively charged analytes to the negatively charged column surface. The mobile phase consists mostly of organic solvent (such as acetonitrile) to minimise the formation of a solvation layer around the charged analytes.
BIST (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple charges. BIST is a simple way to achieve retention and separation for applications that are traditionally difficult or impossible to achieve with any other HPLC columns.
Features
- BIST provides an additional separation tool to a whole class of organic and inorganic charged molecules
- BIST A has a negative surface charge and is useful for the analysis of anions
- BIST requires a doubly charged buffer ion present in the mobile phase that are opposite in charge to that of the stationary phase surface
- Requires reduced water in the mobile phase to minimise ion solvation
- BIST range also includes BIST A+ for more retention of negatively charged molecules, and BIST B and BIST B+ for analyis of positively charged molecules
- Compatible with LC-MS
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specifications
| BIST A | |
| Mode | BIST (Bridge Ion Separation Technique) |
| Surface Charge | Negative |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm, 10 µm |
| Pore Size | 100 Å and 300 Å |
| Temperature Range | 0 - 50°C |
| Pressure Limit | 345 bar |
