emp BIOTECH ZetaSep FPLC Desalting Columns

ZetaSep FPLC desalting columns efficiently separate large biomolecules like proteins and nucleic acids from small impurities such as salts, dyes, and other contaminants, while enabling buffer exchange. These columns offer simple, rapid, and reproducible separation, ideal for post-purification desalting or buffer exchange. They handle sample sizes of 200 µL (1 mL column) and 1 mL (5 mL column), effectively removing low-molecular-weight impurities from biomolecules larger than 5 kDa. Featuring Zetadex-25 Superfine, a high-flow, chemically stable size-exclusion matrix, ZetaSep desalting columns provide excellent resolution with minimal buffer and pH effects.

Part Number Product Description Price Qty
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Features

  • Ideal for desalting, buffer exchange, and removal of low-molecular-weight contaminants from larger biomolecules
  • Available in 1 mL and 5 mL volumes accommodating different sample sizes and purification needs
  • Efficient separation of molecules above 5 kDa from smaller impurities, ensuring high purity of the target biomolecules
  • Simple, rapid and reproducible separation using a syringe, pump or liquid chromatography system 
  • Zetadex-25 superfine resin provides high flow rates, excellent resolution and chemical stability

Specifications

  ZetaSep Desalt
Bed Volume 1 mL 5 mL
Size of Eluted Proteins > 5kDa > 5kDa
System Compatibility - Automated liquid chromatography systems (MPLC, FPLC, ÄKTA)
- Peristaltic pump
- Syringe
- Automated liquid chromatography systems (MPLC, FPLC, ÄKTA)
- Peristaltic pump
- Syringe
Column Ports Inlet 10 – 32 (1/16”) female
Outlet 10 – 32 (1/16”) male
Inlet 10 – 32 (1/16”) female
Outlet 10 – 32 (1/16”) male
Support Matrix Zetadex Z25 Zetadex Z25
Grade Superfine Superfine
Recommended Flow Rates 0.5 - 2 mL 1 - 5 mL
Size of Eluted Proteins > 5kDa > 5kDa

Literature

User guide for the ZetaSep Desalt FPLC columns
Solutions for downstream processing