CentriPure Gravitational Columns from emp BIOTECH
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CentriPure gravitational columns from emp BIOTECH are advanced gel filtration columns designed for rapid purification, desalting, and buffer exchange of biological samples using gravity flow. Filled with Zetadex Medium gel, these columns provide a faster, more efficient alternative to dialysis or tangential flow filtration (TFF) for removing small molecules like salts, dyes, and biotin from larger proteins, nucleic acids, or nanoparticles.
Available with two grades of Zetadex - Zetadex-25 for proteins larger than 5 kDa and nucleic acids longer than10 bases, and Zetadex-50 for proteins larger than 25 kDa and nucleic acids longer than 20 bases - these columns process fixed sample volumes with a 1.5-fold dilution and achieve over 85% recovery. They can be pre-washed with water for desalting or pre-equilibrated with a buffer of choice for a customised buffer exchange, making them versatile for a range of applications.
Features
- Suitable for rapid purification, desalting and buffer exchange using gravity flow
- Twelve column sizes available from 0.2 mL sample volume up to 150 mL
- Two grades of Zetadex are available, Zetadex-25 and Zetadex-50, which have distinct separation characteristics arising from different degrees of cross-linking
- Ultrapure gel and specially treated sinter frits ensure outstanding resolution, low cross-contamination and high selectivity
- Removal efficiency of small molecules typically exceeds 99.99%, with a dilution of around 1.5 to 2-fold, and a nucleotide or protein recovery of greater than 85%
- Gravity flow operation makes CentriPure columns simple and easy to use, completing processes in under 15 minutes
- CentriPure Lab racks make purification easy and convenient
Specifications
| Zetadex-25 | Zetadex-50 | |
| Grade | Medium | Medium |
| Particle Size | 50 – 150 μm | 50 – 150 μm |
| System Compatibility | Gravity flow | Gravity flow |
| Proteins | Larger than 5 kDa (MWCO) | Larger than 25 kDa (MWCO) |
| Oligonucleotides | Longer than 10 base pairs/nucleotides | Longer than 20 base pairs/nucleotides |
| Spheroidal nanoparticles | Greater than 2 nm Ø | Greater than 4 nm Ø |
