HALO® BioClass ES-C18 HPLC Columns from Advanced Materials Technology
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HALO® BioClass ES-C18 Capillary HPLC Columns from AMT
With both tailored particle and pore size options, HALO® BioClass ES- C18 offers specific solutions for protein and peptide applications.
- HALO 1000 Å Protein ES-C18 2.7 µm - Recommended for monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with MWs ≤ 500 kDa
- HALO 400 Å Protein ES-C18 3.4 µm - Recommended for proteins and polypeptides < 500 kDa
- HALO 160 Å Peptide ES-C18 - Recommended for peptides and polypeptides < 20 kDa. Available in 2, 2.7 and 5 µm
With both tailored particle and pore size options, HALO® BioClass ES- C18 offers specific solutions for protein and peptide applications.
- HALO 1000 Å Protein ES-C18 2.7 µm - Recommended for monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with MWs ≤ 500 kDa
- HALO 400 Å Protein ES-C18 3.4 µm - Recommended for proteins and polypeptides < 500 kDa
- HALO 160 Å Peptide ES-C18 - Recommended for peptides and polypeptides < 20 kDa. Available in 2.7 and 5 µm.
HALO® BioClass ES- C18 columns from Advanced Materials Technology provide specific solutions for protein and peptide applications with tailored particle and pore size options.
For protein analysis the HALO® ES- C18 columns are available in a 1000 Å 2.7 µm particle and a 400 Å 3.4 µm particle. The 1000 Å particle is used for the ultimate resolution of mAbs and other large proteins, while the 400 Å particle is designed for fast analysis of these compounds at lower back pressures.
For peptide applications the HALO® ES-C18 160 Å columns are specifically designed to offer selectivity, stability, and reproducibility to address complex peptide separations. The columns are excellent options for both fast and high resolution UHPLC separations, have high peak capacity and are extremely stable at high temperatures and low pH. For peptide characterization, the 160 Å pore size is ideal for small protein fragments and peptides.
Features
- HALO® 160 Å ES-C18 columns are ideal for tryptic digests, post-translational modifications (PTMs), polypeptides applications
- HALO® 400 Å and 1000 Å are ideal for mAb analysis and offer best in class resolution for full characterisation
- Superficially porous particle technology for fast, high resoltuon separations
- High peak capacities delivering rugged, reliable performance for use with either UHPLC, HPLC, or LC-MS
- High temperature stability phases
- USP L1 designation
Specifications
| HALO® 160 Å ES-C18 | HALO® 400 Å ES-C18 | HALO® 1000 Å ES-C18 | |
| Phase | Diisobutyloctadecylsilane | Diisobutyloctadecylsilane | Diisobutyloctadecylsilane |
| Particle Type | Fused-Core® | Fused-Core® | Fused-Core® |
| Particle Size | 2 µm, 2.7 µm, 5 µm | 3.4 µm | 2.7 µm |
| Pore Size | 160 Å | 400 Å | 1000 Å |
| Endcapped | No | Yes | Yes |
| Low pH/Temperature Limit | 1/90⁰C | 1/90⁰C | 1/90⁰C |
| High pH/Temperature Limit | 8/40⁰C | 8/40⁰C | 8/40⁰C |
| Hardware | Stainless Steel | Stainless Steel | Stainless Steel |
| USP Designation | L1 | L1 | L1 |
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