SiELC Promix AP Columns
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SiELC Promix AP columns are suitable for the analysis of small and medium size peptides under 3 kDa with a PI value below 7.0. This mixed-mode analytical column has negatively charged acidic ion-pairing groups embedded within long ligand chains.
Promix AP is designed for analysis of short basic peptides. This phase allows the retention of peptides with several histidine and lysine residues. A pH gradient is the most convenient way to control retention of basic peptides. It is recommended to use a buffer with a neutral or slightly acidic pH (4.5 – 7) in the beginning of the gradient and use a more acidic buffer (pH 2 – 3.5) at the end of the gradient elution.
Features
- Stationary phase for small and medium size peptides under 3 kDa with pI value below 7.0
- Reverse phase and ion-exchange mechanism of interaction
- Use acidic mobile phase pH from 2 to 5.5
- Use ion strength of at least 20 mM
- 100 Å and 300 Å pore sizes are available
- Completely scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
- Promix range also includes Promix MP for medium sized peptides (1 to 10 kDa) and Promix SP for small peptides under 1 kDa with pI value below 6.0
Specifications
| Primesep AP | |
| Phase Type | Mixed-mode |
| Particle Type | Fully Porous |
| Particle Size | 5 µm |
| Pore Size | 100 Å and 300 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
Literature
Unsurpassed selectivity and peak capacity for peptides and proteins