Thermo Scientific Acclaim 300 C18 Columns
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Acclaim 300 C18 Biochromatography Columns from Thermo Scientific
Achieve high-resolution peptide mapping and protein separations with Thermo Scientific Acclaim 300 C18 LC Columns.
The 3 µm ultrapure silica particles reduce the limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns for faster separations.
Thermo Scientific Acclaim 300 C18 columns are designed for the separation of proteins, peptides, and other biological macromolecules. This line of columns extends the same unique bonding chemistry developed for the Acclaim 120 columns to a wide-pore silica substrate. The Acclaim 300 columns consist of 3 µm diameter silica particles with 300 Å diameter pores, bonded with ahighly characterized C18 phase. The unique bonding chemistry results in a high-density, highly uniform phase coverage with extensive end-capping. The use of a 3 µm silica particle accelerates the diffusion of the mobile phase into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µm column packings, it is possible to increase the flow rate of the mobile phase and run shallower gradients on shorter columns to achieve the desired separation in a shorter time period.
Features
- High-performance C18 bonding chemistry on 300 Å pore silica
- Application tested for suitability in peptide mapping
- Reproducible for dependable results
- Extremely low bleed, fully compatible with MS
- Stable between pH 2 .5 and 7.5
Specifications
| Acclaim 300 C18 | |
| Bonded Phase | C18 |
| Particle Type | Fully Porous |
| Particle Size | 3 µm |
| Pore Size | 300 Å |
| Surface Area | 100 m2/g |
| Carbon Load | 8% |
| Maximum Temperature | 60°C |
| pH Range | 2.5 - 7.5 |
| USP Designation | L1 |
