YMC-Triart Bio C4 Columns
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YMC-Triart Bio C4 Biochromatography HPLC Columns
YMC-Triart Bio C4 is a novel, organic/inorganic hybrid silica derivatized with C4 (L26 USP designation) based on wide-pore (300Å) particles. This new stationary phase is designed for HPLC and UHPLC separations of proteins and oligonucleotides, and is particularly useful for intact antibody analyses run at high temperatures. Triart Bio C4 is well-suited for high-sensitivity analysis using mass spec-friendly mobile phases containing formic acid and/or TFA. As with all Triart products, chromatographers enjoy wide temperature and pH ranges (up to 90°C at pH 1-7, and 50°C at pH 7-10), allowing these parameters to be used as tools for optimizing separation conditions, without having to worry about stationary phase durability.
YMC-Triart Bio C4 is a novel, organic/inorganic hybrid silica derivatized with C4 (L26 USP designation) based on wide-pore (300 Å) particles. This new stationary phase is designed for HPLC and UHPLC separations of proteins and oligonucleotides, and is particularly useful for intact antibody analyses run at high temperatures. Triart Bio C4 is well-suited for high-sensitivity analysis using mass spec-friendly mobile phases containing formic acid and/or TFA. As with all Triart products, chromatographers enjoy wide temperature and pH ranges (up to 90°C at pH 1-7, and 50°C at pH 7-10), allowing these parameters to be used as tools for optimizing separation conditions, without having to worry about stationary phase durability.
Features
- Triart Bio C4 is a wide-pore 300 Å hybrid silica phase designed for biomolecule separations
- Suitable for the separation of intact antibodies under high temperatures (up to 90°C)
- Available in UHPLC and HPLC columns packed with 1.9, 3, or 5 µm particles, offering a wide pH range of 1-10
- Compatible with 100% aqueous mobile phases
- Proprietary endcapping makes for excellent peak shapes of proteins when using MS-friendly mobile phases containing formic acid and TFA
- The chemical stability of the Triart material combines with the inherent mechanical durability of the particles themselves to contribute to long column lifetime
- Rigorous manufacturing controls result in legendary YMC column-to-column and lot-to-lot reproducibility
Specifications
| YMC-Triart Bio C4 | |
| Bonded Phase | C4 |
| Particle Type | Fully Porous |
| Bonding | Polymeric Type |
| Endcapped | Yes |
| Particle Size | 1.9 µm, 3 µm, 5 µm |
| Pore Size | 30 nm / 300 Å |
| Temperature Limit | pH 1 - 7: 90°C, pH 7 - 12: 50°C |
| pH Range | 1 - 10 |
| AQ Stability | 100% H2O eluents |
| USP Designation | L26 |
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