CHROMATOGRAPHY WEBCAST ARCHIVE
Green HPLC: The Critical Role of Efficiency - Feb 2024
Event Overview
Chromatographers have a moral and commercial responsibility to reduce the environmental impact of our work in separation science. Amongst the 12 principles of Green Analytical Chemistry (GAC), reduction in solvent usage and waste is perhaps the easiest to achieve in HPLC via reduced dimension columns and superficially porous chromatography media. This webcast explains the underlying theory, presents relevant worked examples and highlights the key strategies to ensure success in reducing solvent volume and the welcome reduction in purchase and disposal costs.
Size Exclusion Chromatography (SEC) - May 2024
Event Overview
Size exclusion chromatography (SEC) is a vital tool in assessing the critical quality attributes of biopharmaceuticals which are critical to ensuring fit for purpose medicines. Webcast attendees will gain a better understanding of the principles and practice of the SEC technique, understand analytical results and have an improved ability to establish, optimize and troubleshoot analytical conditions.
HPLC Stationary Phase Selection - Aug 2024
Event Overview
How do you choose stationary phases for HPLC method development?
In this webcast we aim to achieve one simple objective, to convey our tips and tricks on improving your chances of separation success with the fewest number of experimental iterations or stationary phases screened. If that sounds appealing, then we’d love you to join us for a discussion on how to select stationary phases and optimize your chromatography during method development.
Practicalities of Hydrogen or Nitrogen as a GC Carrier Gas - Nov 2024
Event Overview
Given recent supply chain issues, increased cost and sustainability issues of Helium, why don’t more laboratories make the change to an alternative carrier gas?
In this webcast, we aim to outline the practical considerations of changing carrier gas, and realizing the cost benefits and security of supply which accompany the change. We also consider the situations in which Nitrogen or Hydrogen can be used as an alternative with minimum disruption to current analytical methodology as well as any potential issues that you may find on making the switch.
Fully vs Superficially Porous Columns: Pros, Cons, and Best Applications - June 2025
Event Overview
For over five decades, fully porous particles (FPP) have been the tried and tested support material underpinning liquid chromatography in a wide range of applications, modes, and geometries. Given recent advances in ultra-high performance liquid chromatography (UHPLC) using sub-2μm particles, this technology has proven that it can deliver extremely efficient and sensitive analyses. On the other hand, superficially porous particles (SPP, core-shell) have steadily risen in popularity since their commercial introduction in 2006.
In this webcast, we explore both FPP and SPP column technologies. We acknowledge the strengths of each particle type and the role they play in modern liquid chromatography, while considering the limitations that might give one particle type an advantage over the other.
Practical Solutions for Oligonucleotide Analysis - August 2025
Event Overview
Chromatographic analysis of Oligonucleotides (OGN) presents many practical challenges and several different approaches need to be taken to achieve full characterization or quantitation of target analytes.
This webcast highlights the potential pitfalls associated with OGN analysis and highlights practical solutions across column chemistry, eluent preparation and the use of passivated (bioinert) hardware. We consider ion-exchange, ion-pairing and non-ion pairing reversed phase and HILIC solutions for OGN analysis and highlight the concept of ‘on-off’ chromatography for high throughput oligonucleotide analysis.