Agilent AdvanceBio RP-mAb Columns

AdvanceBio RP-mAb HPLC Columns from Agilent

Agilent AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications.

Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization.

Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible results. Available in a range of chemistries – SB-C8, C4, and diphenyl.

Agilent AdvanceBio RP-mAb columns are designed to optimize the performance of intact and reduced mAb analysis when analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications. Analysis of intact and reduced monoclonal antibodies are critical measurements for characterizing therapeutic proteins and understanding their efficacy and stability. Poor chromatographic separations can result in rework and even compromise the accuracy of the characterization. 

Based on Poroshell technology with unique engineering for pore size and bonded phases, AdvanceBio RP-mAb columns deliver higher resolution and faster run times to provide accurate, reproducible analysis and results. These RP-mAb columns are available in a range of chemistries – SB-C8, C4, and diphenyl.

Part Number Product Description Price Qty
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Features

  • 3.5 µm superficially porous particles with 450 Å wide pores increase mAb resolution while maintaining compatibility with all LC instruments
  • Shorter analysis times compared to columns packed with fully porous particles of the same size
  • Robust Poroshell packed bed and 2 µm inlet frit extend column lifetime by helping prevent inlet blockage
  • Flexible method development with range of chemistries including SB-C8, C4, and diphenyl

Specifications

  AdvanceBio RP-mAb C4 AdvanceBio RP-mAb SB-C8 AdvanceBio RP-mAb Diphenyl
Bonded Phase C4 SB-C8 Diphenyl
Particle Type Superficially Porous Superficially Porous Superficially Porous
Particle Size 3.5 µm  3.5 µm  3.5 µm
Pore Size 450 Å 450 Å 450 Å
Temperature Limit 90°C 90°C 90°C
pH range 1 - 8 1 - 8 1 - 8
Pressure Limit 600 bar 600 bar 600 bar

Related Posts

Literature

User guide for Altura, AdvanceBio and Agilent Bio reversed phase HPLC columns
Your essential resource for biomolecule analysis

Resolve mAbs faster and better with Agilent AdvanceBio RP-mAb columns

Collection of Agilent app notes for intact and subunit analysis
Better characterization of biomolecules using Agilent AdvanceBio reversed phase columns