AdvanceBio Surfactant Profiling Columns from Agilent

AdvanceBio Surfactant Profiling HPLC columns are designed to quickly and reproducibly characterise non-ionic surfactants and their degradation products, particularly in biologics. The unique selectivity of this reversed phase column allows for excellent separation and high resolution of the various components of the surfactants. The 3.5 μm particle size allows for robustness with samples in heavy matrix such as formulation buffer, while maintaining excellent separations.

Every batch of the AdvanceBio Surfactant Profiling media is tested with a mix of a polysorbate and its major fatty acid to ensure resolution and reproducibility. With regulatory bodies increasing scrutiny on polysorbate degradation and its adverse effects, it’s prudent to proactively incorporate this analysis in development labs now. The AdvanceBio Surfactant Profiling HPLC column is the best-in-class column to characterise degradation in polysorbates and other surfactants.

Part Number Product Description Price Qty
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Features

  • Selectivity allows for rapid and high resolution separations
  • AdvanceBio Surfactant Profiling media batches are tested with a relevant application to ensure high performance
  • Recommended operating temperature 25 30°C, maximum 80°C
  • Compatibile with water and all common organic solvents - avoid tetrahydrofuran (THF)

Specification

  AdvanceBio Surfactant Profiling
Particle Type Fully Porous
Particle Size 3.5 µm
Maximum Temperature 80°C
pH Range 1 8
Pressure Limit 400 bar (50 mm) / 600 bar (100, 150 mm) 
Hardware Stainless Steel

Videos

From Degradation to Distinction: A Novel and Complete Characterisation of Polysorbates, presented by Wendi Hale, PhD - Agilent Biocolumns Global Product Manager.

This video describes a novel method to characterise polysorbate degradation quickly and easily.

Literature

In depth characterisation of surfactant and surfactant degradation in biotherapeutic applications
Analytical HPLC columns for surfactant degradation profiling