Altura ZORBAX Eclipse Plus C18 Ultra Inert Columns from Agilent
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Altura HPLC columns from Agilent set a new standard for bio-inert liquid chromatography, eliminating signal loss and peak distortion caused by metal–analyte interactions. Featuring Agilent’s proprietary Ultra Inert coating, these columns retain the strength and pressure tolerance of stainless steel hardware while delivering a completely inert flow path. This prevents nonspecific adsorption, improves quantitation accuracy, and enhances chromatographic reproducibility. The result is consistently sharper peaks, higher sensitivity, and greater stability – ideal for metal-sensitive or complex biomolecules in demanding LC and LC-MS workflows.
The Altura ZORBAX Eclipse Plus C18 combines the trusted performance of Agilent’s ZORBAX Eclipse Plus C18 media with the exceptional inertness of Altura column hardware. Recommended for peptide mapping, sequence confirmation, and post-translational modification (PTM) monitoring analysis of protein therapeutics, it delivers superior peptide recovery, resolution, and signal response in complex digest mixtures. Using the same 1.8 µm particle technology as the ZORBAX Eclipse Plus RRHD small-molecule columns, the Ultra Inert hardware minimises peptide adsorption and signal suppression, improving signal-to-noise ratios and the detection of low-abundance PTMs. Designed for LC/MS peptide mapping workflows, the Altura ZORBAX Eclipse Plus C18 ensures reliable sequence coverage, accurate quantitation, and robust analytical performance.
Features
- Ultra Inert Flow Path – Prevents metal–peptide interactions for greater recovery and reproducibility
- Proven ZORBAX Media – Uses trusted Eclipse Plus C18 chemistry with 1.8 µm particles for high-resolution separations
- Optimised for Peptide Mapping – Delivers consistent performance for complex protein digests and PTM analysis
- Enhanced Sensitivity – Provides higher signal-to-noise ratios and improved detection of low-abundance modifications
- Superior Peak Shape – Ensures sharp, symmetrical peaks for accurate quantitation
- Robust and Durable – Maintains performance under high pressure and extended use
- LC-MS Ready – Ideal for sequence confirmation and structural characterisation of protein therapeutics
Specifications
| Altura ZORBAX Eclipse Plus C18 | |
| Bonded Phase | C18 |
| Particle Type | Fully Porous |
| Particle Size | 1.8 µm |
| Pore Size | 95 Å |
| Surface Area | 160 m2/g |
| Maximum Temperature | 60°C |
| pH Range | 2 - 9 |
| Pressure Limit | 1200 bar |
| Hardware | Ultra Inert Stainless Steel |
