Agilent PL-SAX Columns
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Agilent PL-SAX columns are ideal for the anion-exchange HPLC separations of proteins, peptides, and deprotected synthetic oligonucleotides under denaturing conditions. The strong anion-exchange functionality, covalently linked to a chemically stable fully porous polymer, extends the operating pH range. In addition, the anion-exchange capacity is independent of pH.
For synthetic oligonucleotides, separations using denaturing conditions of temperature, organic solvent, and high pH are all possible. PL-SAX delivers improved chromatography for self-complementary or G-rich sequences that may associate to form aggregates or hairpin structures. The 5 μm material provides high-efficiency separations of n and n-1 sequences.
A wide range of particle sizes and column geometries permits analysis scale-up to purification. The strong anion-exchange functionality provides a material with exceptional chemical and thermal stability, even with sodium hydroxide eluents, leading to long column lifetime.
Features
- Anion-exchange purifications over a wider pH range extend applications
- HPLC flow rates and rapid equilibration reduce purification cycle times
- Small particles deliver excellent chromatographic performance
- Wide range of particle sizes and two pore sizes for flexible analysis to scale up purification
- 1000 Å pore size for high-capacity purifications
- 4000 Å gigaporous particles with improved mass transfer for large biomolecules and high-speed, high-resolution purifications
- Exceptional stability for long column lifetime
Specification
| PL-SAX | |
| Phase | Strong Anion Exchange |
| Particle Type | Fully Porous PS/DVB particles |
| Particle Size | 5 µm, 8 µm, 10 µm, and 30 µm |
| Pore Size | 1000 Å and 4000 Å |
| pH Range | 1 – 14 |
| Maximum Temperature | 80°C |
| Hardware | Stainless Steel |
