Agilent Poroshell 300 Columns
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Poroshell 300 HPLC Columns from Agilent
Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks.
Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. But Poroshell columns use a unique particle made with a layer of porous silica on a solid core of silica.
This reduces the diffusion distance for proteins making practical rapid HPLC separations of peptides and proteins up to 500 – 1,000 kDA. Poroshell 300SB C18, C8, C3, and 300Extend columns provide excellent stability and selectivity choices with TFA and formic acid mobile faces.
Poroshell 300Extend C18 columns can be used at varying pH levels for unique separations and additional selectivity choices.
Agilent Poroshell 300 columns are ideal for fast separations of proteins and peptides because the 5 μm superficially porous particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. Poroshell columns with StableBond bonded phases provide excellent stability and selectivity choices with TFA and formic acid mobile phases. The Poroshell 300Extend-C18 column can be used from pH 2 - 11 for unique separations. These columns can also be used for analytical protein separations and LC/MS separations.
Peptides and proteins are typically separated slowly to reduce the potential peak broadening of these slow diffusing analytes. However, Poroshell columns use a superficially porous particle made with a thin layer of porous silica, 0.25 μm thick, on a solid core of silica. This reduces the diffusion distance for proteins, making rapid HPLC separations of peptides and proteins up to 500 – 1000 kDa possible with 400/600 bar HPLC systems, including the Agilent 1260 Infinity II bio-inert LC.
Features
- UHPLC separations of biomolecules with superficially porous particles
- 300 Å pore provide high efficiency and recovery with proteins (up to 1000 kDa) and monoclonal antibodies
- Achieve long lifetime at low pH with Poroshell 300SB; at high pH with Poroshell 300Extend-C18
- Optimize recovery and selectivity with four different bonded phases – 300SB-C18, 300SB-C8, 300SB-C3, and 300Extend-C18
Specifications
| Poroshell 300SB-C18 | Poroshell 300SB-C8 | Poroshell 300SB-C3 | Poroshell 300Extend-C18 | |
| Bonded Phase | C18 | C8 | C3 | C18 |
| Particle Type | Superficially Porous | Superficially Porous | Superficially Porous | Superficially Porous |
| Endcapped | No | No | No | Yes |
| Particle Size | 5 µm | 5 µm | 5 µm | 5 µm |
| Pore Size | 300 Å | 300 Å | 300 Å | 300 Å |
| Temperature Limit | 90°C | 90°C | 90°C | 40°C above pH 8, 60°C below pH 8 |
| pH Range | 1 - 8 | 1 - 8 | 1 - 8 | 2 - 11 |
| USP Designation | L1 | L7 | L56 | L1 |
