Agilent ZORBAX GF-250 and GF-450 SEC Columns
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Agilent ZORBAX GF-250 and GF-450 SEC columns are for size separations of proteins and other biomolecules. The separation range is 4,000 - 900,000 for globular proteins when using GF-250 and GF-450 columns in series. The GF-250 and GF-450 columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.
The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation. Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins.
Features
- High efficiency and reproducibility with short analysis time
- Semi-prep and prep column dimensions
- Compatible with organic modifiers and denaturants
- Wide usable pH range of 3 - 8
- L35 USP designation
Specifications
| ZORBAX GF250 | ZORBAX GF450 | |
| Pore Size | 150 Å | 300 Å |
| Particle Type | Fully Porous | Fully Porous |
| Molecular Weight Lower Limit | 4000 Da | 10,000 Da |
| Molecular Weight Upper Limit | 400,000 Da | 900,000 Da |
| Temperature Limit | 60°C | 60°C |
| pH range | 3 - 8.5 | 3 - 8.5 |
| Hardware | Stainless Steel | Stainless Steel |
| USP Designation | L35 | L35 |
