Thermo Scientific DNAPac PA200 and DNAPac PA200RS Columns
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Thermo Scientific DNAPac PA200 and DNAPac PA200RS columns offer the highest resolution charge-based oligonucleotide separations. These anion-exchange columns provide unsurpassed resolution of full length from n–1, n+1 (and other failure sequences). Compatible with LC-UV, these columns offer orthogonal separation to reversed phase columns, separating the oligonucleotide sample by size and charge.
The 8µm DNAPac PA200 is optimal for conventional HPLC users. Those looking for UHPLC separations, or running more complicated samples will prefer the 4µm PEEK-lined stainless steel DNAPac PA200RS for the highest resolution separation.
Features
- Achieve n, n-1 resolution for oligonucleotides
- Resolve oligonucleotides with secondary structures
- Assay phosphorothioate purity
- Selectivity control with eluent pH, salt, and solvent
- Resolve RNA with aberrant (2' – 5') links from normal SSRNA
- Available in both standard HPLC and rapid separation (RS) UHPLC formats
- DNAPac PA200RS packed in bioinert PEEK-lined stainless steel column bodies
Specifications
| DNAPac PA200 | DNAPac PA200RS | |
| Phase | Strong Anion Exchange | Strong Anion Exchange |
| Particle Type | Polymeric | Polymeric |
| Particle Size | 8 µm | 4 µm |
| Pore Size | Non-porous | Non-porous |
| pH Range | 2.5 - 12.5 | 2.5 - 12.5 |
| Maximum Temperature | 85°C | 85°C |
| Hardware | 85°C | PEEK-lined Stainless Steel |
| Pressure Limit | 275 bar | 690 bar |