puriFlash BIO Prep HPLC Columns for Peptides from Advion-Interchim Scientific
Main image
Click to view image in fullscreen
![]()
All pictures shown are for illustration purpose only. Actual product may vary.
puriFlash BIO preparative HPLC columns from Advion-Interchim Scientific are purpose-built for the purification of peptides, delivering high resolution, strong recovery, and consistent reproducibility across diverse peptide workflows. Based on optimised reversed phase chemistries and available in multiple pore sizes and ligand types – including C18, C8 and C4 – these columns provide tailored selectivity for peptides ranging from short sequences to large biomolecules. With column diameters from 4.6 mm to 50 mm and particle sizes of 5 µm, 10 µm or 15 µm, the puriFlash BIO series supports seamless scale-up from analytical evaluation to preparative-scale purification.
Phase Options Tailored for Peptide Purification
- puriFlash BIO 100 C18-N: A non-endcapped monofunctional C18 phase (100 Å) designed for in-process QA/QC of peptide synthesis.
- puriFlash BIO 100 C18-T: A one-step endcapped tri-functional C18 phase (100 Å) suited to the analysis and purification of peptides under 40 amino acids and up to ~5 kDa.
- puriFlash BIO 100 C18-XS: A multi-step endcapped monofunctional C18 phase (100 Å) optimised for mid- to non-polar peptides, including hydrophobic peptides under 40 amino acids and up to ~5 kDa.
- puriFlash BIO 200 C18-N: A non-endcapped monofunctional C18 phase (200 Å) suitable for polar peptides up to 160 amino acids (~20 kDa) under pseudo-HILIC conditions, as well as hydrophobic peptides under 80 amino acids (~10 kDa).
- puriFlash BIO 200 C18-T: A one-step endcapped tri-functional C18 phase (200 Å) designed for mid- to non-polar and hydrophobic peptides under 80 amino acids and up to ~10 kDa.
- puriFlash BIO 200 C18-XS: A multi-step endcapped monofunctional C18 phase (200 Å) intended for mid-polar to non-polar peptides under 80 amino acids (~10 kDa), with stability suitable for operation under basic conditions up to pH 10.
- puriFlash BIO 200 C8-N: A non-endcapped monofunctional C8 phase (200 Å) supporting polar peptides up to 160 amino acids (~20 kDa) under pseudo-HILIC conditions, and hydrophobic peptides under 80 amino acids (~10 kDa).
- puriFlash BIO 300 C4-AQ: A hydrophilic C4 phase (300 Å) developed for large peptides, natural peptides, and fatty acids larger than ~80 amino acids and up to ~100 kDa.
Features
- Specialised Reversed-Phase Chemistries – Offers C18, C8 and C4 ligands to match peptide hydrophobicity and molecular size
- Multiple Pore Sizes – Ensures optimised mass transfer for short peptides through to large biomolecules
- High Resolution and Recovery – Designed for sharp separations and efficient collection of peptide products
- Scalable Formats – Available in 4.6–50 mm ID and 5 µm, 10 µm or 15 µm particle sizes for smooth method scale-up
- Reproducible Performance – Delivers consistent retention and selectivity across demanding purification workflows
- Extended Column Lifetime – Guard cartridges, when used with the appropriate guard holder, provide extra protection to maximise the life of your prep column
Specification
| C18-N | C18-T | C18-XS | C8-N | C4-AQ | |
| Particle Type | Fully Porous | Fully Porous | Fully Porous | Fully Porous | Fully Porous |
| Particle Size | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm |
| Pore Size | 100 Å, 200 Å | 100 Å, 200 Å | 100 Å, 200 Å | 200 Å | 300 Å |
| Endcapped | None | One-Step | Multi-Step | None | Mixed |
| pH Range | 1.5 - 8.0 | 1.5 - 8.0 | 1.0 - 10.0 | 1.5 - 8.0 | 1.5 - 8.0 |
Literature
puriFlash and Uptisphere Bio silicas are specifically designed to meet the requirements of preparative liquid chromatography
The essential systems and consumables for purification, evaporation, and mass spectrometry
