Agilent AdvanceBio Peptide Mapping Columns

AdvanceBio Peptide Mapping HPLC Columns from Agilent

Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence.

These advanced biocolumns are superficially porous and have a 120Å pore size which is optimum for the analysis of the peptides produced by enzymatic digest of proteins.

The 2.7 µm particle size enables UHPLC performance on HPLC systems, which delivers high resolution separations 2–3 times faster than with fully porous HPLC columns. Each batch of the AdvanceBio Peptide Mapping media is tested with a peptide mix to ensure suitability and reproducibility, and to provide greater confidence in the analytical data generated.

Use Agilent’s 10-Peptide Quality Control Standard, the same standard Agilent uses to QC its columns, to evaluate your column’s performance over its lifetime. It can be used for HPLC or LC/MS. Approximately 20 injections per vial. Order part number 5190-0583.

Agilent AdvanceBio Peptide Mapping columns are designed to provide high-resolution peptide maps for protein identification and determination of post translation modifications. These reversed phase columns provide the ability for you to quickly resolve and identify amino acid substitutions/modifications in a protein primary sequence. These advanced biocolumns are superficially porous and have a 120 Å pore size which is optimum for the analysis of the peptides produced by enzymatic digest of proteins. The 2.7 µm particle size enables UHPLC performance on HPLC systems, which delivers high resolution separations 2 to 3 times faster than with fully porous HPLC columns.

Each batch of the AdvanceBio Peptide Mapping media is tested with a peptide mix to ensure suitability and reproducibility, and to provide greater confidence in the analytical data generated. Use Agilent’s 10-Peptide Quality Control Standard (part number 5190-0583), the same standard Agilent uses to QC its columns, to evaluate your column’s performance over its lifetime.

AdvanceBio Peptide Mapping columns are the first members of Agilent’s growing state-of-the-art family of bio columns. They are designed to deliver consistent, exceptional performance for the separation and characterization of peptides and proteins.

Part Number Product Description Price Qty
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Features

  • High-resolution peptide maps for protein identification and determination of post translation modifications
  • 2.7 µm superficially porous particles give you UHPLC performance on any 400 bar HPLC instrument
  • Achieve increased MS sensitivity with formic acid mobile phases on any HPLC
  • 2.7 µm superficially porous particles give you UHPLC performance on any 400 bar HPLC instrument

Specifications

  AdvanceBio Peptide Mapping
Bonded Phase C18
Particle Type Superficially Porous
Particle Size 2.7 µm
Pore Size 120 Å
Temperature Limit 60°C
pH Range 2 - 8
Pressure Limit 600 bar

 

Literature

Your essential resource for biomolecule analysis
Reduce peptide mapping time without losing resolution
Optimize your peptide mapping separations to achieve the best possible results
Collection of Agilent app notes on peptide mapping
Better characterization of biomolecules using Agilent AdvanceBio reversed phase columns
Workflow solutions for both intact protein and peptide mapping for identification and localization of PTMs