Avantor® ACE® HPLC Column Method Development Kits

Avantor® ACE® method development kits are available in a wide range of dimensions for rapid, systematic method development. Each kit contains carefully selected ACE phases which enables the power of selectivity to be fully exploited. Screening columns containing different bonded phases under the same mobile phase conditions can help you achieve your desired separation more quickly, therefore reducing method development time.

Avantor® ACE® Advanced method development kits contain ACE C18, ACE C18-AR, and ACE C18-PFP phases. Ideal kit for starting method development and is particularly recommended for compounds containing aromatic rings.

Avantor® ACE® Extended method development kits contain ACE SuperC18, ACE C18-Amide, and ACE CN-ES phases. Use ACE SuperC18 to exploit selectivity changes at low, intermediate, and high pH. ACE C18-Amide and ACE CN-ES phases offer alternative selectivity, especially for polar molecules.

Avantor® ACE® UltraCore method development kits contain ACE UltraCore SuperC18 and SuperPhenylHexyl phases. Ultra-inert core-shell particles and Encapsulated Bonding Technology (EBT) provide excellent peak shape.

Avantor® ACE® HILIC method development kits contain ACE HILIC-A, HILIC-B, and HILIC-N phases. Alternative and improved selectivity to reversed-phase for polar and very polar analytes.

Part Number Product Description Price Qty
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Features

Avantor® ACE® Advanced Method Development Kits

  • Contains ACE C18, ACE-C18-AR, and ACE-C18-PFP phases
  • ACE C18 remains the "go-to" column of choice for HPLC and UHPLC separations and provides a rugged, reproducible starting point for method development
  • ACE C18-AR combines the excellent performance and advantages of the ACE C18 phase with the added selectivity of an integral phenyl group
  • ACE C18-PFP bring together the stability, reproducibility and low bleed of the ACE C18 phase with the additional selectivity of an integral PFP group
  • Available in 1.7, 2, 3 and 5 μm paricle sizes
  • HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit

 

Avantor® ACE® Extended Method Development Kits

  • Contains ACE SuperC18, ACE C18-Amide, and ACE-CN-ES phases
  • ACE SuperC18 is a uniquely bonded, EBT endcapped C18 phase which offers excellent inertness, efficiency and durability over an extended pH rane of 1.5 - 11
  • ACE C18-Amide is a unique polar-embedded phase that offers enhanced retention and resolution of polar acidic, phenolic and hydroxy-subsituted analytes
  • ACE CN-ES is a unique phase having an extended alkyl chain with a terminal cyano group for increased retention and stability
  • Available in 1.7, 2, 3 and 5 μm paricle sizes
  • HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit

 

Avantor® ACE® UltraCore Method Development Kits

  • Contains ACE UltraCore SuperC18 and SuperPhenylHexyl phases
  • Use to exploit selectivity changes at low, intermediate and high pH
  • Ultra-inert core-shell particles and Encapsulated Bonding Technology (EBT) provide excellent peak shape
  • Available in 2.5 and 5 μm paricle sizes
  • HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit

 

Avantor® ACE® HILIC Method Development Kits

  • Contains ACE HILIC-A, HILIC-B, and HILIC-N phases
  • ACE HILIC-A, ACE HILIC-B and ACE HILIC-N provide alternative selectivity to each other
  • ACE HILIC-A is an acidic character phase with an ionisable negative surface charge depending on mobile phase pH
  • ACE HILIC-B is a basic character phase with an ionisable positive surface charge depending on mobile phase pH
  • ACE HILIC-N is a neutral character phase capable of H-bonding amongst other mechanisms of interaction
  • Available in 1.7, 3 and 5 μm paricle sizes
  • HPLC/UHPLC hardware format with 1,000 bar / 15,000 psi pressure limit

Specifications

Avantor® ACE® Advanced Method Development Kits

  Avantor® ACE® C18 Avantor® ACE® C18-AR Avantor® ACE® C18-PFP
Functional Group Octadecyl Octadecyl with integral phenyl Octadecyl with integral PFP
USP Listing L1 L1 L1
Endcapped Yes Yes Yes
Particle Size 1.7, 2, 3, 5 µm 1.7, 2, 3, 5 µm 1.7, 2, 3, 5 µm
Pore Size 100 Å 100 Å 100 Å
Surface Area 300 m2/g 300 m2/g 300 m2/g
Carbon Load 15.5% 15.5% 14.3%
pH Range 2 - 8 2 - 8 2 - 8
100% Aqueous Compatible - Yes Yes

 

 

Avantor® ACE® Extended Method Development Kits

  Avantor® ACE® SuperC18 Avantor® ACE® C18-Amide Avantor® ACE® CN-ES
Functional Group Octadecyl encapsulated Polar embedded amide Cyano with extended alkyl spacer
USP Listing L1 L1/L60 L10
Endcapped Yes Yes Yes
Particle Size 1.7, 2, 3, 5 µm 1.7, 2, 3, 5 µm 1.7, 2, 3, 5 µm
Pore Size 90 Å 100 Å 100 Å
Surface Area 400 m2/g 300 m2/g 300 m2/g
Carbon Load 14.8% 16.4% 12.6%
pH Range 1.5 - 11.5 2 - 8 2 - 8
100% Aqueous Compatible - Yes Yes

 

 

Avantor® ACE® UltraCore Method Development Kits

  Avantor® ACE® UltraCore SuperC18
Avantor® ACE® UltraCore SuperPhenylHexyl

Functional Group Octadecyl encapsulated Phenyl hexyl encapsulated
USP Listing L1 L11
Endcapped Encapsulated Encapsulated
Particle Size 2.5 µm 5 µm 2.5 µm 5 µm
Pore Size 95 Å 95 Å
Surface Area 130 m2/g 100 m2/g 130 m2/g 100 m2/g
Carbon Load 7% 5.4% 4.6% 3.6%
pH Range 1.5 - 11 1.5 - 11
100% Aqueous Compatible - -

 

Avantor® ACE® HILIC Method Development Kits

  Avantor® ACE® HILIC-A Avantor® ACE® HILIC-B Avantor® ACE® HILIC-N
Functional Group Proprietary SIL Proprietary aminopropyl Proprietary polyhydroxy
USP Listing L3 L8 Pending
Endcapped No No No
Particle Size 1.7, 3, 5 µm 1.7, 3, 5 µm 1.7, 3, 5 µm
Pore Size 100 Å 100 Å 100 Å
Surface Area 300 m2/g 300 m2/g 300 m2/g
Carbon Load N/A 4% 7%
pH Range 2 - 7 2 - 7 2 - 7
100% Aqueous Compatible - - -

Literature

Intelligent solutions for method development
Specifications of the Avantor ACE columns
Product catalogue containing the full range of Avantor ACE columns