HELIX Amaze Mixed-Mode HPLC Columns

HELIX Amaze TH mixed-mode HPLC columns contain HILIC, cation exchange, and anion exchange functionalities within one ligand attached to the silica surface. This enables the separation of polar neutral, polar acidic, polar basic, hydrophobic acidic, and hydrophobic basic compounds in one method. By changing the pH of the mobile phase, the strength of basic and acidic groups can be adjusted allowing retention of weakly ionisable groups. HILIC separation with lower concentration of acetonitrile is possible due to the highly polar and ionisable groups on the surface. Optimisation of the separation of critical pairs of compounds can be achieved by changing the amount of organic, buffer pH, buffer concentration, and type of buffer.