HALO® RP-Amide HPLC Columns from Advanced Materials Technology
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HALO® RP-Amide Analytical HPLC Columns from AMT
The one-step bonded, endcapped, amide-based, polar embedded stationary phase of HALO 90 Ã… RP-Amide provides a stable, reversed phase packing with decreased hydrophobic character. HALO 90 Ã… RP-Amide can be used for basic, acidic, or neutral compounds. HALO 90 Ã… RP-Amide columns will have similar retention but different selectivity for some compounds compared to alkane phases. Phenols and anilines interact more strongly with the amide phase compared to C18/C8. Typically, bases move to shorter retentions compared to C18 columns. Non-polar solutes show little differences.
Available in 3 particle sizes:
- HALO® 2 µm columns can meet or exceed the performance of most non-core sub-2 µm columns at pressures one-third to one-half the back pressure under the same conditions
- HALO® 2.7 µm columns can meet or exceed the performance of most non-core sub-2 µm columns at pressures one-third to one-half the back pressure under the same conditions
- HALO® 5 µm columns match the performance of totally porous 3 µm columns at roughly half the back pressure under the same conditions
HALO® RP-Amide HPLC columns from Advanced Materials Technology have a polar embedded stationary phase that is advantageous for the reversed phase separation of polar compounds. Increased retention and improved peak shapes for basic compounds are observed compared to C18 phases. HALO® RP-Amide offers alternative selectivity to traditional alkyl phases making it an excellent addition to method development screening kits. Available with 2 µm, 2.7 µm, and 5 µm Fused-Core® particles for use on either HPLC or UHPLC instruments.
Features
- Polar embedded stationary phase for reversed phase separations of polar compounds
- Suitable for analysis of basic compounds such as alcohols, acids, phenols and catechins
- Provides alternative selectivity to alkyl stationary phases
- 100% aqueous compatible
- LCMS compatible with minimum column bleed
- Meets USP L60 specifications
- Available with 2 µm, 2.7 µm, and 5 µm Fused-Core® particles
Specifications
| HALO® RP-Amide | |
| Bonded Phase | C16 Amide |
| Particle Type | Fused-Core® |
| Particle Size | 2 µm, 2.7 µm, 5 µm |
| Pore Size | 90 Å |
| Endcapped | Yes |
| Low pH/Temperature Limit | 2/60°C |
| High pH/Temperature Limit | 9/40°C |
| Pressure Limit | 2 µm - 1000 bar 2.7 µm and 5 µm - 600 bar |
| Hardware | Stainless Steel |
| USP Designation | L60 |
