puriFlash BIO Prep HPLC Columns for Oligonucleotides from Advion-Interchim Scientific
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puriFlash BIO RP-NH preparative columns from Advion-Interchim Scientific are designed for the efficient purification of oligonucleotides. By integrating both reversed phase and ion-exchange functionalities, the stationary phase – functionalised with alkyl chains and amine groups – provides enhanced selectivity and optimal separation efficiency. With two pore sizes tailored to different oligonucleotide lengths, the BIO RP-NH series offers precise resolution for short sequences as well as larger biomolecules. Columns are available from 4.6 mm to 50 mm internal diameter and in 5 µm, 10 µm or 15 µm particle sizes, enabling scalable purification from analytical development through to preparative production.
Phase Options Designed for Oligonucleotide Purification
- puriFlash® BIO 200 RP-NH: Developed for the analysis and purification of oligonucleotides up to 40 mers, offering reliable resolution and consistent performance for short to medium-length sequences.
- puriFlash® BIO 300 RP-NH: Optimised for larger oligonucleotides, including aptamers and DNA constructs, providing strong retention and high-quality purification of higher-molecular-weight biomolecules.
Features
- Dual-Mode Selectivity – Combines reversed-phase and ion-exchange properties for enhanced control over oligonucleotide separations
- Tailored Pore Sizes – Provides optimised resolution for both short oligos and larger biomolecules
- High Separation Efficiency – Functionalised with alkyl chains and amines to ensure sharp peak shapes and consistent performance
- Scalable Formats – Available from 4.6 mm to 50 mm ID with 5 µm, 10 µm or 15 µm particle sizes for seamless scale-up
- Robust and Versatile – Suitable for diverse oligonucleotide purification workflows, from R&D to preparative-scale production
Specification
| puriFlash BIO 200 RP-NH | puriFlash BIO 300 RP-NH | |
| Mode | Mixed-Mode | Mixed-Mode |
| Particle Type | Fully Porous | Fully Porous |
| Particle Size | 5 µm, 10 µm, 15 µm | 5 µm, 10 µm, 15 µm |
| Pore Size | 200 Å | 300 Å |
| Surface Area | 150 m²/g | 100 m²/g |
| Carbon Load | 4% | 2% |
| Endcapped | No | No |
| pH Range | 1.5 - 8.0 | 1.5 - 8.0 |
