Agilent Polaris Semi-Prep
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Polaris SemiPrep/Preparative LC Columns from Agilent
Polaris columns have been designed for high aqueous conditions. The combination of high phase density bonding, ultra pure silica, and silanol shielding leads to excellent peak shape among polar-modified columns.
Polaris C18-A columns offer alternate selectivity for general polar applications and are designed with hydrogen-bond accepting endcapping.
Polaris C18-Ether columns are endcapped with an ether group to create a more polar surface for selectivity variation.
Polaris Amide-C18 columns provide subtle alternative selectivity because they do not have steric protection. They utilize an embedded amide, similar to Bonus-RP columns.
Polaris C8-A offers an alternative selectivity to standard C8 phases and has a lower hydrophobicity than Polaris C18-A, making it ideal for polar samples, for faster overall analysis times.
Polaris C8-Ether columns are endcapped with an ether group to create a more polar surface for selectivity variation.
Polaris Silica (Si) and Amino (NH2) columns are ideal for retention of polar columns in normal phase mode.
Features
- Excellent chromatography with mixtures of polar and nonpolar analytes
- Various unique selectivities
- Resilient Polaris NH2 phase offers rapid analysis of carbohydrates and extended lifetime compared to other NH2 phases
Specifications
Polaris C18 Specifications
| Polaris C18-A | Polaris C18-Ether | Polaris Amide C18 | |
| Pore Size | 180 Å | 180 Å | 180 Å |
| Surface Area | 200 m2/g | 200 m2/g | 200 m2/g |
| Carbon Load | 13.8% | 12.1% | 15% |
| Endcapped | Yes | Yes | Yes |
| Pore Volume | 1.1 cm3/g | 1.1 cm3/g | 1.1 cm3/g |
| Ligand Coverage | 3.9 µmol/m2 | 3.3 µmol/m2 | 4.4 µmol/m2 |
| USP Designation | L1 | L1 | L60 |
Polaris C8 Specifications
| Polaris C8-A | Polaris C8-Ether | |
| Pore Size | 180 Å | 180 Å |
| Surface Area | 200 m2/g | 200 m2/g |
| Carbon Load | 7.4% | 7.1% |
| Endcapped | Yes | Yes |
| Pore Volume | 1.1 cm3/g | 1.1 cm3/g |
| Ligand Coverage | 4.8 µmol/m2 | 4.5 µmol/m2 |
| USP Designation | L7 | L7 |
Polaris Normal Phase Specifications
| Polaris NH2 | Polaris Si-A | |
| Pore Size | 180 Å | 180 Å |
| Surface Area | 200 m2/g | 200 m2/g |
| Carbon Load | 5.5% | - |
| Endcapped | Amide | - |
| Pore Volume | 1.1 cm3/g | 1.1 cm3/g |
| Ligand Coverage | 3.8 µmol/m2 | |
| USP Designation | L8 | L3 |
Literature
Overview of Agilent's range of prep columns for small molecules and biomolecules