Porvair Assay Plates
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Porvair assay plates are specifically designed for absorbance, fluorescence, and luminescence applications and are available in 24, 96 or 384 well formats. The plates are made from pure polystryrene for low background to enhance the accuracy and sensitivity of results. The plates are moulded in either clear, black, or white colours and are available with solid bottoms, or assembled with clear polystyrene, glass, quartz or COC bases. These can then be further treated to create specific surface characteristics for cell culture or assay applications.
Features
- ANSI-SBS Footprint
- Compatible with automated systems
- Uniform external dimensions and tolerances
- For top reading instruments use a solid white plate for absorbance measurements and solid black plate for fluorescence readings
- For bottom reading instruments use clear bottomed plates
- Visible wavelength range (900 - 350 nm) measurements require clear plastic bases
- Readings between 220 - 350 nm require a UV-transparent material such as quartz or cyclo-olefin co-polymer (COP/COC)
- Optical glass sheet is used where visible range detection is combined with confocal optics or whole plate imaging
- Manufactured from polystyrene with no additives
- Flat bottoms give high surface areas, round bottoms for good mixing, or V-wells for high liquid recovery
Specifications
Absorbance Assays
| Application | Recommended Plate | |
| Top Reading Instruments | High signal from majority of wells e.g. test kits, ELISA | Use simple solid clear plates |
| Low signal from some or all wells e.g. kinetics or genetic marker assays | Use solid white plates to boost the signal | |
| Bottom Reading Instruments | Normal visualisation | Use white plates with clear plastic bottoms |
| Confocal visualisation | Use white plates with glass bottoms | |
| For very sensitive assays | Use zero crosstalk white plates with individual clear wells |
Fluorescence Assays
| Application | Recommended Plate | |
| Top Reading Instruments | Use solid black plates | |
| Bottom Reading Instruments | Visible wavelength range 350-700 nm, high signal from majority of wells e.g. GFP, Reporter Gene kits | Use black clear-bottomed plates |
| Low signal from some or all wells e.g. poor sensitivity assays, low specificity | Use black Zero crosstalk clear bottom plates to boost the signal to noise | |
| UV wavelength range 220-350 nm | Use black clear-bottomed COP plates | |
| Confocal or whole plate imaging | Use black glass-bottomed plates |
Luminescence Assays
| Application | Recommended Plate | |
| Top Reading Instruments | Good signal | Use solid white plates |
| Poor signal or high dynamic range across the plate | Use solid black plates with white wells | |
| Bottom Reading Instruments | Use white clear-bottomed plates |
Literature
Guide to help you choose the correct assay plate
Comprehensive range of precision-assembled, top quality microplates to enhance your research and analysis