CentriPure MINI Columns from emp BIOTECH

CentriPure MINI columns from emp BIOTECH are compact gel filtration columns designed for rapid desalting, buffer exchange, and removal of small molecular weight impurities from proteins, oligonucleotides, and nanoparticles. These conical, ready-to-use columns process sample volumes up to 100 µL in less than 5 minutes using a centrifuge, making them perfect for quick lab workflows. Packed with Zetadex-25 or Zetadex-50 gel, CentriPure MINI columns efficiently remove over 99.99% of contaminants such as salts, dyes, and biotin while ensuring high recovery of target molecules. With options pre-equilibrated in water, PBS, TRIS, or azide-stabilized buffers, these columns offer flexibility for various applications, providing a fast and effective alternative to traditional desalting methods.

Part Number Product Description Price Qty
product image

Features

  • Rapid processing - purifies, desalts, and exchanges buffers in under 5 minutes
  • Ready-to-use, sterile-packed columns with an easy centrifugation protocol
  • Conical columns handle sample volumes up to 100 µL
  • Available with Zetadex-25 (5 kDa cut-off) or Zetadex-50 (25 kDa cut-off) for different purification needs
  • Columns available pre-washed with pure water, PBS, TRIS, or stabilised with sodium azide
  • Removes over 99.99% of salts, dyes, haptens, and other small molecules
  • Ensures excellent recovery of proteins, nucleic acids, and nanoparticles

Specifications

  Desalt Z-25 Desalt Z-25AZ SEQ Z-50 TRIS Z-50 TRIS Z-25 PBS Z-50 PBS Z-25
Gel Matrix Zetadex-25SF Zetadex-25SF Zetadex-50SF Zetadex-50SF Zetadex-25SF Zetadex-50SF Zetadex-25SF
Sample Buffer Deionized water Deionized water and 0.02% sodium azide Deionized water 1 mM TRIS, pH 8 1 mM TRIS, pH 8 Standard PBS, pH 7 Standard PBS, pH 7
Application For desalting of proteins larger than 5 kDa, nucleic acids longer than 10 bp/nt, or nanoparticles > 2 nm Ø For desalting of proteins > 5 kDa, nucleic acids > 10 bp/nt, or nanoparticles > 2 nm Ø, and simultaneous elution into aqueous 0.02% sodium azide For desalting of oligonucleotides longer than 20 bp/nt from Sanger sequencing reactions For purification of proteins larger than 25 kDa or nanoparticles > 4 nm Ø, and simultaneous buffer exchange to TRIS (1 mM, pH 8) For purification of proteins larger than 5 kDa or nanoparticles > 2 nm Ø, and simultaneous buffer exchange to TRIS (1 mM, pH 8) For purification of immunoglobulins and other proteins larger than 25 kDa and simultaneous buffer exchange to PBS (8 mM, pH 7) For purification of proteins larger than 5 kDa or nanoparticles > 2 nm Ø, and simultaneous buffer exchange to PBS (8 mM, pH 7)
Gel Bed Volume 0.5 mL 0.35 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL
Sample Volume 2 to 100 μL* 2 to 100 μL* 2 to 100 μL* 2 to 100 μL* 2 to 100 μL* 2 to 100 μL* 2 to 100 μL*
Optimal Centrifuge Conditions 1000 x g for 2 min 1000 x g for 2 min 1000 x g for 2 min 1000 x g for 2 min 1000 x g for 2 min 1000 x g for 2 min 1000 x g for 2 min
Removal of Dye (50 μL 1mM 5/6 carboxyfluorescein in 0.1 M NaHCO3) >99.99% >99% >99.999% >99.999% >99.95% (TAMRA dye substituted for fluorescein) >99.999% >99.99%
Removal of Dye (100 μL 1mM 5/6 carboxyfluorescein in 0.1 M NaHCO3) >99.5% Not recommended to use samples >50 μL >99.95% >99.99% >99.5% (TAMRA dye substituted for fluorescein) >99.99% >99.5%
Removal of Salt (50 μL 0.8 M NaCl) >99.5% Not evaluated due to sodium azide >99.9% N/A N/A N/A N/A
Removal of Salt (100 μL 0.8 M NaCl) >99.0% Not evaluated due to sodium azide >99.0% N/A N/A N/A N/A

* optimised for 50 μL

Literature

For rapid purification, desalting, and buffer exchange
Solutions for downstream processing