ZetaSep Activated FPLC Columns from emp BIOTECH
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ZetaSep activated FPLC columns from emp BIOTECH are designed for the immobilisation of ligands, enabling targeted purification tailored to specific biomolecules. With a choice of covalent binding technologies, ZetaSep activated FPLC columns offer customisable solutions, allowing users to select the most suitable strategy for ligand attachment. ZetaSep Activated FF6 FPLC columns are based on highly cross-linked 6% agarose and are available in two different options for ligand immobilisation.
- ZetaSep Epoxy-activated FF6 binds ligands such as sugars or carbohydrates via hydroxyl groups, or other molecules via primary amine or thiol groups.
- ZetaSep Aldehyde-activated FF6 binds primary amines, such as lysine residues on proteins, through reductive amination, forming stable secondary amine linkages for enhanced durability in purification processes.
Features
- Activated ZeraSep FPLC columns for immobilisation of ligands pursuant to targeted purification
- Offers a variety of covalent binding options to suit different purification strategies
- Stable cross-linked agarose matrix provides a robust platform with high chemical stability for reliable performance
- Allows precise control over purification conditions for enhanced target molecule recovery
- Available 1 mL and 5 mL FPLC columns for use with chromatography systems
- Also available as Zetarose activated bulk resins
Specifications
| Zetarose Aldehyde-activated FF6 | Zetarose Epoxy-activated FF6 | |
| Activation Density | >5 μmol/mL | >75 μmol/mL |
| Binds to | -NH2 | -NH2 -SH -OH |
Literature
Solutions for downstream processing