GL Sciences MonoSpin SPE Spin Columns
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MonoSpin solid phase extraction (SPE) spin columns from GL Sciences deliver rapid and efficient sample preparation through advanced silica monolith technology. Unlike traditional packed columns, MonoSpin uses a disk-shaped silica monolith with uniform continuous pores, providing high liquid permeability, a large surface area, and excellent recovery. Designed for biological samples such as blood, serum, and urine, MonoSpin spin columns enable fast extraction, purification, and concentration by centrifugation. Its frit-free design ensures consistent flow, minimal sample loss, and reliable performance, even with small sample volumes or trace analytes.
The MonoSpin SPE spin column series is available in multiple formats: Type S (50–800 µL) for small-volume samples, Type L (0.5–8 mL) for larger volumes, and a 96-well plate for high-throughput workflows.
To suit a wide range of analytical applications, MonoSpin columns are offered in several chemistries:
- Reversed Phase: C18, C18 FF, and Ph
- Ion Exchange: SAX, SCX, C18-AX, C18-CX, and CBA
- HILIC: NH2 and Amide
- Affinity: PBA, ME, TiO, Phospholipid, and Trypsin HP
- Antibody Purification: ProA, ProG, and ProL
Combining ease of use, speed, and high recovery, MonoSpin solid phase extraction spin columns are an advanced, reliable solution for modern analytical and bioanalytical sample preparation.
Features
- Centrifuge Operation – Simple and convenient solid phase extraction using standard laboratory centrifuges
- Rapid Sample Processing – High flow rates through the monolithic silica structure enable fast extraction and purification
- Excellent Reproducibility – Reliable performance even with elution volumes of 100 µL or less (Type S)
- User-Friendly Design – Easy-to-operate format for routine and specialised analytical workflows
- Flexible Formats – Available as Type S (50–800 µL) for small volumes, Type L (0.5–8 mL) for larger samples, and 96-well plate for high-throughput applications
- Optimised for Small Volumes – Ideal for limited or trace sample quantities without dilution
- Versatile Selectivity – Broad choice of functional chemistries for targeted purification and enrichment
Specification
| Reversed Phase | |
| MonoSpin C18 | Octadecyl functional group; ideal for drug extraction from biological samples and for desalting and enriching peptide samples |
| MonoSpin C18 FF | Octadecyl functional group with large through-pore monolithic silica; optimised for high-viscosity biological samples requiring efficient drug extraction and peptide enrichment |
| MonoSpin Ph | Phenyl functional group; suitable for recovering hydrophobic drugs in biological samples with weaker retentivity and alternative selectivity to C18 |
| Ion Exchange | |
| MonoSpin SAX | Trimethylaminopropyl bonded phase; combines strong anion exchange and weak hydrophobic interactions for the extraction of acidic drugs |
| MonoSpin SCX | Benzenesulphonic acid bonded phase; combines strong cation exchange and hydrophobic interactions, ideal for the extraction of basic drugs |
| MonoSpin C18-AX | Mixed-mode octadecyl and trimethylaminopropyl phase; provides strong retention in high-salt serum samples and efficient recovery of acidic drugs |
| MonoSpin C18-CX | Mixed-mode octadecyl and benzenesulphonic acid phase; enhances clean-up efficiency and is optimal for dissociated basic drugs in biological samples |
| MonoSpin CBA | Carboxylic acid bonded phase; provides weak cation exchange properties, ideal for extracting basic drugs |
| HILIC | |
| MonoSpin NH2 | Aminopropyl bonded phase; suitable for enriching sugar chains and hydrophilic compounds under HILIC mode |
| MonoSpin Amide | Amide bonded phase; designed for extracting sugar chains and various hydrophilic acidic and basic compounds using HILIC separation |
| Affinity | |
| MonoSpin PBA | Phenyl boronic acid functional group; enables selective extraction of cis-diols such as catecholamines |
| MonoSpin ME | Iminodiacetic acid bonded phase; specialised for the recovery of trace metal ions |
| MonoSpin TiO | Titanium dioxide-coated monolith; excellent for adsorbing and removing phospholipids from biological samples |
| MonoSpin Phospholipid | Titanium dioxide and zirconium dioxide-coated monolith; provides high efficiency in phospholipid adsorption and removal |
| MonoSpin Trypsin HP | Trypsin-immobilised monolithic silica; performs rapid and highly efficient protein digestion in approximately 15 minutes |
| Antibody Purification | |
| MonoSpin ProA | Immobilised with Protein A on a silica monolith modified with a hydrophilic polymer, enabling rapid and efficient purification of antibodies with high recovery and minimal non-specific adsorption |
| MonoSpin ProG | Immobilised with Protein G on a hydrophilic polymer-modified silica monolith, providing fast and high-yield purification of antibodies while reducing unwanted protein binding |
| MonoSpin ProL | Immobilised with Protein L ligand (KANEKA KanCap™ L), offering strong affinity for the variable region of immunoglobulin kappa light chains and enabling purification of antibodies and their fragments such as scFv, Fab, and F(ab’)2 |
