HALO® BioClass C4 HPLC Columns from Advanced Materials Technology
All pictures shown are for illustration purpose only. Actual product may vary.
With both tailored particle and pore size options, HALO® BioClass C4 offers application specific solutions for proteins. The densely bonded, extensively endcapped dimethylbutyl stationary phase of HALO® C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds such as proteins.
HALO 1000 Å C4 is based on a wide-pore 1000 Å Fused-Core® particle design. This particle exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 µm thin porous shell and the small overall particle size of 2.7 µm.
HALO 400 Å C4 is based on a wide-pore 400 Å Fused-Core® particle design. This particle exhibits very high column efficiency due to the shallow diffusion paths in the 0.2 µm thin porous shell and the small overall particle size of 3.4 µm.
HALO® BioClass C4 columns from Advanced Materials Technology offer application specific solutions for proteins with tailored particle and pore size options. The densely bonded, extensively endcapped dimethylbutyl stationary phase of HALO® C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds.
The HALO® C4 columns are available in a 1000 Å 2.7 µm Fused-Core® particle and a 400 Å 3.4 µm Fused-Core® particle. The 1000 Å particle is used for the ultimate resolution of mAbs, while the 400 Å particle is designed for fast analysis of large proteins at lower back pressures.
Features
- HALO® 1000 Å C4 is ideal for mAb analysis and offers best in class resolution for full characterization
- HALO® 400 Å C4 is suitable for large protein analysis and is well suited for high speed separations
- Alternative selectivity to HALO® ES-C18 and HALO® Diphenyl phases
- USP L26 designation
- Available particle sizes of 2.7 µm for 1000 Å pore size and 3.4 µm for 400 Å pore size
Specifications
| HALO® 400 Å C4 | HALO® 1000 Å C4 | |
| Phase | Dimethylbutylsilane | Dimethylbutylsilane |
| Particle Type | Fused-Core® | Fused-Core® |
| Particle Size | 3.4 µm | 2.7 µm |
| Pore Size | 400 Å | 1000 Å |
| Endcapped | Yes | Yes |
| Low pH/Temperature Limit | 2/90⁰C | 2/90⁰C |
| High pH/Temperature Limit | 9/40⁰C | 9/40⁰C |
| Hardware | Stainless Steel | Stainless Steel |
| USP Designation | L26 | L26 |
