HPLC OPERATOR TRAINING COURSE
This HPLC operator course provides a step-by-step approach to setting up and performing an HPLC analysis.
Using a typical HPLC-UV method, we carefully work through the key aspects of reviewing the method, preparing mobile phase, setting up the instrumentation, running the sequence and basic interpretation of results.
With a strong focus on good laboratory practice, this HPLC operation training equips you with the skills to minimise errors, reduce downtime, and improve overall efficiency.
Ideal for new HPLC operators or those with limited experience, the course focuses on building confidence and competence in running HPLC methods effectively. The primary aim is to establish reliable working practices, with minimal explanation of the HPLC process.
HPLC OPERATOR COURSE DETAILS
- An understanding of each of the parameters in a typical HPLC method
- How these parameters work in practice
- How to use this knowledge to setup an HPLC system and method in a way that minimises errors and maximises efficiency
We offer flexible delivery options for this instructor-led course:
- On-site: One full-day session at your laboratory or any location of your choice.
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Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.
No prior knowledge is required for this course.
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Method Review and Workflow Planning
- Overview of the reversed phase HPLC process
- Typical method parameters
- Instrumentation / equipment
- Capturing the process in a lab book
- Calculations for reagents and standards
- System settings
- Checklist
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Solution Preparation
- Buffer, salts and hydrates
- pH adjustment
- Mixing and filtration
- Sample preparation
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System Set-up
- System checks
- Priming the system
- Fittings and tubing
- Column installation
- System equilibration
- Order of analysis, sequence
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Running the Sequences
- What tests should we perform
- Error reduction during vialing up
- Pre-sequence checks
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Results and Basic Calculations
- Integration
- Introduction to System suitability
- Single point (proportional) calibration
- Calculations
- Reporting
