LC-MS FOR OLIGONUCLEOTIDES - INTACT MOLECULE
TRAINING COURSE
Oligonucleotide therapeutics continue to expand rapidly, and with them the need for analysts who can confidently interpret, troubleshoot, and optimise LC-MS workflows. LC-MS for Oligonucleotides – Intact Molecule is a comprehensive, application-focused course designed to give you the structural understanding and analytical skills needed to characterise intact oligos with accuracy and confidence.
This course guides you from foundational molecular concepts through to real-world mass spectrometry acquisition and data interpretation
This course is ideal for analytical scientists, mass spectrometrists, and lab personnel involved in oligonucleotide R&D, bioanalysis, or QC - particularly those who are expanding into large molecule analysis, or needing to characterize or sequence oligo-based materials.
LC-MS FOR OLIGONUCLEOTIDES - INTACT MOLECULE COURSE DETAILS
- Nucleotide Architecture: Learn the core building blocks - sugars, phosphates, and bases - and how they define oligo structure and MS behaviour
- Oligonucleotide Formation & Mer Weights: Understand how oligos are assembled and how mer additions contribute to overall molecular mass
- Ionisation & Charge State Formation: Grasp ESI fundamentals, multiple charging, and how deprotonation and adducts shape complex spectra
- Adduct Chemistry (−H and +Adducts): See how proton loss and adduct gain combine to produce mixed charge states critical for accurate deconvolution
- Molecular Weight Determination: Determine mass using non-HRAM and HRAM approaches, including conventional deconvolution, C13 delta analysis, and isotope pattern recognition
- In-Source CID & Pseudo-MS³: Learn how controlled fragmentation and pseudo-MS³ improve structural confidence
- Chromatography for Oligonucleotide LC-MS: Get the essentials of MS-compatible oligo chromatography, including ion-pairing, solvents, and separation strategies
- Workshops: Learn to apply all the taught techniques in carefully designed graded workshops that allow you to practice as you learn
We offer flexible delivery options for this instructor-led course:
- On-site: One full-day session at your laboratory or any location of your choice.
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Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.
Experienced in small molecule LC-MS.
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Nucleotide structure, formation (sugar, phosphate, base)
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Oligonucleotide formation and mer weights within the oligo molecule
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Ionisation and Multiple Charging
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Ionisation by combination of multiple -H and +adducts
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Molecular weight deconvolution (from non HRAM instruments)
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Isotopes
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Molecular weight deconvolution from C13 delta in HRAM instruments
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In-source CID
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Pseudo MS3 acquisition
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Oligonucleotide chromatography for ms acquisition (optional)
