OPTIMISING SAMPLE PREPARATION

1 DAY COURSE

Ensure that you pick the right sample prep technique and learn how to get the best of it

Sample preparation training can help you learn or brush up on different techniques such as Liquid/Liquid Extraction (LLE), QuEChERS and Solid Phase Extraction (SPE), among others, no matter your level of experience. Learn the absolute essentials and method development tips and tricks and apply this knowledge for the betterment of your lab workflow.

A comprehensive one-day course designed to increase expertise and optimise method development time for all users of sample preparation and SPE in particular. 

The course is backed with a host of explanatory images and multimedia material from our range of e-learning products. Practical examples are included to reinforce the learning experience.

COURSE OVERVIEW

Who is this course for?

This course is for LC or GC analysts or method developers who need to master the principles of major sample preparation techniques, including Solid Phase Extraction, to design and implement effective protocols.

 

Previous Knowledge

Knowledge of chromatography and a basic grounding in chemistry are beneficial. Previous experience of sample preparation and SPE will also be advantageous.

 

What you will learn

  • The need for sample preparation
  • The range of techniques available for sample clean-up
  • Liquid/liquid Extraction (LLE) and Supported Liquid Extraction (SLE)
  • QuEChERS
  • Phospholipid Removal
  • The basics of SPE and practical considerations
  • Sorbent Selection, retention mechanisms and generic protocols
  • What are the various extraction techniques offered by SPE
  • Method development and protocol troubleshooting
  • Instrumentation considerations

 

Related Content

 

 

COURSE OUTLINE

The need for sample preparation

  • Choosing a sample preparation technique
  • Dilution, filtration and centrifugation

 

Liquid/Liquid Extraction (LLE)

  • Basic principles
  • Major benefits and limitations
  • Tips and tricks for method development
  • Supported Liquid Extraction (SLE): Benefits and generic protocols

 

QuEChERS

  • Phospholipd Removal
  • Solid Phase Extraction (SPE)
  • SPE Sorbent Substrates
  • Protocol Steps in SPE
  • Hydrophobic or Non-Polar Groups
  • Polar Groups
  • Ionic Groups
  • Chelating Groups

 

Molecular Properties

  • Hydrophobic or Non-Polar Groups
  • Polar SPE
  • Ionic Groups
  • Chelating Groups

 

SPE Mechanisms

  • Non-polar SPE
  • Polar SPE
  • Cation-Exchange SPE
  • Anion-Exchange SPE
  • Mixed-Mode SPE

 

SPE Method Development

  • Analyte Assessment
  • Mechanism Selection
  • Sorbent Screening
  • Procedure Optimization

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