SiELC BIST B+ HPLC Columns
All pictures shown are for illustration purpose only. Actual product may vary.
BIST B+ from SiELC is a positively charged silica-based anion-exchange HPLC column with hydrophilic properties. BIST B+ provides increased retention using similar conditions for positively charged molecules when compared to BIST B. Alternatively, it can be used with lower concentration of the organic modifier in the mobile phase to achieve results comparable to BIST B.
BIST (Bridge Ion Separation Technique) is an LC separation technique that expands the scope of HPLC applications, especially for compounds with multiple charges. BIST is a simple way to achieve retention for compounds that are traditionally difficult to separate. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase.
Features
- BIST provides an additional separation tool to a whole class of organic and inorganic charged molecules
- BIST B+ has a positive surface charge and is useful for the analysis of cations
- BIST requires a doubly charged buffer ion present in the mobile phase that are opposite in charge to that of the stationary phase surface
- Requires reduced water in the mobile phase to minimise ion solvation
- BIST range includes BIST B for less retention of positively charged molecules, and BIST A and BIST A+ for analyis of negatively charged molecules
- Compatible with LC-MS
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specifications
| BIST B+ | |
| Mode | BIST (Bridge Ion Separation Technique) |
| Surface Charge | Positive |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm, 10 µm |
| Pore Size | 100 Å and 300 Å |
| Temperature Range | 0 - 50°C |
| Pressure Limit | 345 bar |
