SiELC Newcrom BH HPLC Columns
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SiELC Newcrom BH is a mixed-mode HPLC column with positively charged basic ion-pairing groups at the terminal end of short ligand chains. This new generation of mixed-mode column moves the charged embedded functional group away from the silica surface enabling uniform surface charge density. Limitations such as restricted use of methanol in the mobile phase, poor peak shape, low efficiency of multi-charged molecules, high buffer concentration requirement and poor retention of non-charged molecules have been overcome with the Newcrom stationary phases.
Newcrom BH is based on spherical silica particles with 100 Å pores and particle sizes of 3 µm and 5 µm. It has a longer ligand chain (25 Å) compared to Newcrom B (100 Å). The longer ligand chains reduce the number of ionic groups over any given surface area, so Newcrom BH has an increased ion capacity compared to Newcrom B.
Features
- Mixed-mode column with positively charged basic ion-pairing groups at the terminal end of short ligand chains (25 Å)
- Improved retention of weak acidic compounds through an anion-exchange mechanism
- Enhanced retention of multi-charged compounds
- Separation of bases by an ion-exclusion mechanism
- Retention of neutral compounds via a reverse-phase mechanism
- Low buffer concentration required to generate efficient ion-exchange
- Compatible with typical mobile phases for LC-MS
- Newcrom range also includes mixed-mode columns Newcrom A, Newcrom AH, and Newcrom B
- Newcrom RI is a special reversed phase column with low silanol activity
- Guard columns connect directly to columns and do not require any additional holders
Specifications
| Newcrom BH | |
| Mode | Mixed-Mode |
| Phase | Positively charged basic ion-pairing groups at the terminal end of short ligand chains (25 Å) |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Range | 1.5 - 7 |
| Pressure Limit | 345 bar |
