SiELC Primesep 100 HPLC Columns
All pictures shown are for illustration purpose only. Actual product may vary.
SiELC Primesep 100 is a mixed-mode HPLC column with acidic ion-pairing groups embedded into long hydrophobic ligand chains. The ligand itself is a C12 chain and its acidic functional group has a pKa of ~1. Reversed phase and cation-exchange functionality are assembled on one molecule to provide good reproducibility and stability.
Primesep 100 is the most versatile column in the entire Primesep family for separating a broad range of compounds. It enhances retention of basic compounds through cation-exchange mechanism, separates acids by ion-exclusion mechanism, and retains neutral compounds by reverse-phase mechanism. The columns are available in all standard dimensions, with 3 µm, 5 µm and 10 µm fully porous particles, and 2.7 µm solid core particles.
Features
- Primesep 100 is a mixed-mode column with acidic ion-pairing groups embedded into long hydrophobic ligand chains
- Available with 3 µm, 5 µm, and 10 µm fully porous particles as well as 2.7 µm solid-core particles
- Most versatile in the entire Primesep family for separating a broad range of compounds
- Enhanced retention of basic compounds through cation-exchange mechanism
- Separates acids by ion-exclusion mechanism
- Retains neutral compounds by reverse-phase mechanism
- Typical mobile phases are based on acetonitrile, water, and an acidic buffer
- Offers mobile phase compatibility with LC-MS
- Resistant to dewetting in 100% aqueous mobile phase
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specification
| Primesep 100 | |
| Mode | Mixed-Mode |
| Phase Type | Acidic ion-pairing groups embedded into long hydrophobic ligand chains |
| Particle Type | Solid Core (2.7 µm) and Fully Porous (3 µm, 5 µm, 10 µm) |
| Particle Size | 2.7 µm, 3 µm, 5 µm, 10 µm |
| Pore Size | 90 Å (2.7 µm) and 100 Å (3 µm, 5 µm, 10 µm) |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
