SiELC Primesep 200 HPLC Columns
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SiELC Primesep 200 is a mixed-mode HPLC column with weak acidic ion-pairing groups embedded into long ligand chains. It provides enhanced retention of strong basic compounds through a cation-exchange mechanism, retains neutral compounds by a reversed phase mechanism, and provides an additional interaction with polar molecules, enabling separation of isomers and structurally related compounds.
Features
- Primesep 200 is a mixed-mode column with weak acidic ion-pairing groups embedded into long ligand chains
- Enhances retention of strong basic compounds through cation-exchange mechanism
- Retains neutral compounds by reverse-phase mechanism
- Provides an additional interaction with polar molecules, enabling separation of isomers and structurally-related compounds
- Typical mobile phases are based on acetonitrile, water, and an acidic buffer
- Offers mobile phase compatibility with LC-MS
- Resistant to dewetting in 100% aqueous mobile phase
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specifications
| Primesep 200 | |
| Mode | Mixed-Mode |
| Phase Type | Weak acidic ion-pairing groups embedded into long ligand chains |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
Literature
Creating new dimensions in the world of chromatography
Solving problems of pharmaceutical HPLC analysis using Primesep columns
New alternative to ion chromatography