SiELC Primesep A HPLC Columns
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SiELC Primesep A is a mixed-mode HPLC column with strong acidic ion-pairing groups embedded in long ligand chains. It provides enhanced retention of weakly basic compounds through a cation exchange mechanism, separates acids by ion-exclusion, and retains neutral compounds by reversed phase mode.
The method development process using Primesep A is simple and versatile. In most cases, separations require a simple mobile phase containing acetonitrile, water and TFA or formic acid. No ion-pairing reagents are required for the retention of ionizable polar analytes. The simple mobile phases allow compatibility with all common detectors (UV, MS, ELSD, and RI).
Features
- Primesep A stationary phase has strong acidic ion-pairing groups embedded in long ligand chains
- Enhances retention of weak basic compounds through cation-exchange mechanism
- Separates acids using ion-exclusion mechanism
- Retains neutral compounds by reversed phase mechanism
- Offers mobile phase compatibility with LC-MS
- Resistant to dewetting in 100% aqueous mobile phase
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specifications
| Primesep A | |
| Mode | Mixed-Mode |
| Phase Type | Strong acidic ion-pairing groups embedded in long ligand chains |
| Particle Type | Fully Porous |
| Particle Size | 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
Literature
Creating new dimensions in the world of chromatography
Solving problems of pharmaceutical HPLC analysis using Primesep columns
New alternative to ion chromatography