SiELC Primesep C HPLC Columns
All pictures shown are for illustration purpose only. Actual product may vary.
SiELC Primesep C is a reverse-phase HPLC column with complex-forming groups embedded on long ligand chains. It improves retention of positively charged compounds by forming host-guest complexes and retains neutral compounds through a reversed phase mechanism. To facilitate the complex formation, the pH of the mobile phase should remain in the range of 3 - 7.
Typical applications include the separation of amines, sulfonium, phosphonium and metal ions. With the complex forming properties of Primesep C columns, primary amines are more retainable than secondary and tertiary amines. This extraordinary elution pattern allows tailoring the separation of amines to provide the most appropriate peak order for quantitation and preparative chromatography.
Features
- Primesep C is a reversed phase column with complex-forming groups embedded on long ligand chains
- Improves retention of positively charged compounds by forming host-guest complexes
- Retains neutral compounds through reverse-phase mechanism
- Unique elution order of positively charged molecules in a chelating HPLC system
- Offers mobile phase compatibility with LC-MS
- Resistant to dewetting in 100% aqueous mobile phase
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specification
| Primesep C | |
| Mode | Mixed-Mode |
| Phase Type | Reverse-phase with complex-forming groups embedded on long ligand chains |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 100 Å |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 7.0 |
| Recommended pH Operating Range | 2.0 - 6.5 |
| Pressure Limit | 345 bar |
