SiELC Primesep SB HPLC Columns
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SiELC Primesep SB is a reverse-phase HPLC column with embedded strongly basic ion-pairing groups. It contains a C18 carbon chain and basic quaternary amine groups. Primesep SB columns provide enhanced retention of acidic compounds through an anion-exchange mechanism, separates hydrophobic basic molecules using a combination of ion-exclusion and reversed phase mechanisms, and retains neutral compounds by a reversed phase mechanism. The columns are available in all standard dimensions, with both fully porous particles and 2.7 µm solid-core particles.
Features
- Primesep SB is a reversed phase column with strong embedded basic ion-pairing groups
- Available with 5 µm and 10 µm fully porous particles as well as 2.7 µm solid-core particles
- Enhanced retention of acidic compounds through an anion-exchange mechanism
- Separates hydrophobic basic molecules using a combination of ion-exclusion and reversed phase mechanisms
- Retains neutral compounds by reversed phase mechanism
- Offers mobile phase compatibility with LC-MS
- Scalable from capillary to prep
- Guard columns connect directly to columns and do not require any additional holders
Specification
| Primesep SB | |
| Mode | Mixed-Mode |
| Phase Type | Reverse-phase column with strong embedded basic ion-pairing groups |
| Particle Type | Solid Core (2.7 µm) and Fully Porous (5 µm and 10 µm) |
| Particle Size | 2.7 µm, 5 µm, 10 µm |
| Pore Size | 90 Å (2.7 µm) and 100 Å (5 µm and 10 µm) |
| Temperature Range | 0 - 50°C |
| pH Limit | 1.5 - 5.0 |
| Recommended pH Operating Range | 2.0 - 4.5 |
| Pressure Limit | 345 bar |
Literature
Creating new dimensions in the world of chromatography
Solving problems of pharmaceutical HPLC analysis using Primesep columns
New alternative to ion chromatography