Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC Columns
Main image
Click to view image in fullscreen
![]()
All pictures shown are for illustration purpose only. Actual product may vary.
Thermo Scientific Acclaim Mixed-Mode HILIC-1 column combines the advantages of reversed phase and HILIC in one column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.
Features
- Separates both polar and non-polar molecules with selectivity complementary to reversed phase columns
- Operates in both reversed phase and HILIC modes
- Retains highly polar molecules
- Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)
- Stable between pH 2.5 and 7.5
- Available in 3 and 5 μm particle sizes with an average pore diameter of 120 Å
- Range also includes Acclaim Mixed-Mode WCX-1 (reversed phase and weak cation exchange) and Acclaim Mixed-Mode WAX-1 (reversed phase and weak anion exchange)
Specification
| Acclaim Mixed-Mode HILIC-1 | |
| Mode | Mixed-Mode |
| Phase Type | Hydrophobic alkyl chain with a diol group at the terminus |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m2/g |
| Temperature Limit | 50°C |
| pH Limit | 2.5 - 7.5 |
| Pressure Limit | 400 bar |
Literature
Introduction to the broad range of chemistries and column families from Thermo Scientific
Practical guide to HILIC mechanisms, method development and troubleshooting
Columns for challenging separation needs
A powerful tool for separating polar molecules
Quick guide to choosing your Acclaim phase by application