Thermo Scientific Acclaim Mixed-Mode WAX-1 HPLC Columns
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Thermo Scientific Acclaim Mixed-Mode WAX-1 HPLC columns provide powerful selectivity control for the separation of anionic molecules. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.
Features
- Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes
- Stationary phase has hydrophobic alkyl chain with an anion terminus
- Selectivity orthogonal to reversed-phase columns
- Powerful selectivity for the separation of difficult mixtures of neutral and anionic analytes
- Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids
- Excellent column efficiency and peak symmetry
- Stable between pH 2.5 and 7.5
- Available in 3 µm and 5 μm particle sizes with an average pore diameter of 120 Å
- USP classification of L78
- Range also includes Acclaim Mixed-Mode WCX-1 (reversed phase and weak cation exchange) and Acclaim Mixed-Mode HILIC-1 (reversed phase and HILIC)
Specification
| Acclaim Mixed-Mode WAX-1 | |
| Mode | Mixed-Mode |
| Phase Type | Hydrophobic alkyl chain with an anion terminus |
| Particle Type | Fully Porous |
| Particle Size | 3 µm, 5 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m2/g |
| Temperature Limit | 50°C |
| pH Limit | 2.5 - 7.5 |
| Pressure Limit | 400 bar |
| USP Designation | L78 |
Literature
Introduction to the broad range of chemistries and column families from Thermo Scientific
Columns for challenging separation needs
Total control of selectivity
User guide for the Acclaim Mixed-Mode WAX-1 columns
Quick guide to choosing your Acclaim phase by application