Thermo Scientific HyperSep Online SPE
All pictures shown are for illustration purpose only. Actual product may vary.
Thermo Scientific HyperSep online SPE products retain specific analytes in a sample matrix when used with an appropriate HPLC column. They have been designed to clean-up and concentrate target analytes in minutes.
HyperSep online SPE products are available in three configurations:
- UNIGUARD Direct-Connect Online SPE Cartridges - convenient reusable product that attaches directly to the switching valve like a HPLC column
- Javelin Direct-Connect Online Columns - finger-tight design for convenience and maximum efficiency
- HPLC Columns for Online SPE - traditional HPLC column fittings and hardware
All products are available with the following HyperSep SPE chemistries:
- HyperSep Retain PEP - for retention of polar and non-polar analytes
- HyperSep Retain CX - for retention of basic and non-polar analytes
- HyperSep Retain AX - for retention of acidic and non-polar analytes
- HyperSep Hypercarb - for retention of extremely polar analytes
Features
- Available in several convenient formats for sample preparation and provide quick and easy connection
- Choose from HyperSep Retain PEP, Retain CX, Retain AX, and Hypercarb phases
- Use these online SPE columns with an appropriate HPLC column and switching valve
- Effective removal of contaminants such as proteins from samples
- Compatible with conventional HPLC systems
- Fast and effective clean-up and concentration of target compounds
Specifications
| Retain PEP | Retain AX | Retain CX | Hypercarb | |
| Phase | Polystyrene DVB material surface modified with urea groups | Polystyrene DVB material partially modified with quaternary amine | Polystyrene DVB material modified with sulfonic acid | 100% porous graphitic carbon |
| Surface area | 550 to 750 m2/g | 550 to 750 m2/g | 550 to 750 m2/g | - |
| Particle size | 40 to 60 µm | 40 to 60 µm | 40 to 60 µm | 30 µm |
| Pore size | 55 to 90 Å | 55 to 90 Å | 55 to 90 Å | 250 Å |
| pH stable | 0 to 14 | 0 to 14 | 0 to 14 | 0 to 14 |
Literature
Brochure containing over 100 applications using HyperSep SPE products