HPLC METHOD DEVELOPMENT
TRAINING COURSE

This instructor-led course provides a comprehensive, process-driven approach to the development of an optimal and robust HPLC method.

For the experienced chromatographer, this course provides a detailed exploration of each critical parameter within a typical method, then builds this into a step-by-step process for method development. We apply relevant theory to underpin a practical approach that maps out the optimal route to a commercial quality method. Importantly, we look at building robustness into each stage of development using a Quality by Design approach, highlighting many common problems and how we develop these out.

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HPLC METHOD DEVELOPMENT COURSE DETAILS

Setting method development objectives
Sample preparation
System choices
Choosing a column and mobile phases
Optimising strategies
Quantitation and system characterisation

We offer flexible delivery options for this instructor-led course:

On-site: One full-day session at your laboratory or any location of your choice.
Online: Two half-day interactive sessions.
View our online training calendar for upcoming dates.

Delegates should have a good knowledge of chromatography and experience as HPLC users. Some experience in method development is recommended. A good grounding in chemistry is also beneficial.

Objectives
- Design specification
- What is QBD?
- Building in robustness
Influencing Resolution
- Retention factor
- Efficiency
- Selectivity
Sample Preparation
- Influencing sample solubility
- Choosing a diluent
Mobile Phase
- Sample polarity
- Organic modifiers
- pH control
- Flowrates
Columns
- Physical dimensions
- Particle type, sizes and UHPLC
- Temperature
- Chemically modified silica
- Reversed phase ligands
- Hydrophobic subtraction model classification
Gradient Elution
- Isocratic or gradient
- Optimising k’
- Effect of each gradient parameter
Building a Practical Process
- Sample preparation
- Retention
- Screening for alpha
- Chromatographic optimisation
- Linearity, range and sensitivity
- Pre-validation